Kuhlemeier C., Strittmatter G., Ward K., Chua N. H.
Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, New York 10021-6399.
Plant Cell. 1989 Apr;1(4):471-478. doi: 10.1105/tpc.1.4.471.
The expression of the pea rbcS-3A gene is responsive to light and restricted to chloroplast-containing cells. Previously, we identified transcriptional elements between -410 and -50 relative to the transcription start site as being sufficient for this regulated expression. Here we investigate the role of the promoter region (-50 to +15) in rbcS-3A expression. We demonstrate an interaction between the promoter and upstream sequences located between -189 and -166, which is important for high level expression. In addition we show that, when the rbcS promoter is driven by a weak test enhancer, it can confer light-responsive but not organ-specific expression upon a bacterial reporter gene.
豌豆rbcS - 3A基因的表达对光有响应,且仅限于含叶绿体的细胞。此前,我们确定了相对于转录起始位点-410至-50之间的转录元件足以实现这种调控表达。在此,我们研究启动子区域(-50至+15)在rbcS - 3A表达中的作用。我们证明了启动子与位于-189至-166之间的上游序列之间存在相互作用,这对高水平表达很重要。此外,我们还表明,当rbcS启动子由一个弱测试增强子驱动时,它可以赋予细菌报告基因光响应性,但不赋予器官特异性表达。