番茄rbcS - 3A基因的表达水平受一个远上游启动子元件以发育调控的方式调节。
Level of expression of the tomato rbcS-3A gene is modulated by a far upstream promoter element in a developmentally regulated manner.
作者信息
Ueda T, Pichersky E, Malik V S, Cashmore A R
机构信息
Department of Biology, University of Pennsylvania, Philadelphia 19104-6018.
出版信息
Plant Cell. 1989 Feb;1(2):217-27. doi: 10.1105/tpc.1.2.217.
Abstract
By Agrobacterium-mediated transformation we have demonstrated that a 1.10-kilobase promoter sequence from the tomato rbcS-3A gene confers light-inducible and organ-specific expression upon fusion to the bacterial chloramphenicol acetyltransferase gene. A biphasic expression profile was obtained by 5' deletion analysis of this promoter, indicating the presence of both positive and negative regulatory elements. A severe reduction in the level of expression was observed when the 5'-terminal 90 base pairs were deleted from the 1.10-kilobase promoter. DNA sequence elements responsible for light inducibility and organ specificity of the gene reside within the -374 base pairs of the proximal part of the promoter and the sequences spanning from -374 to -205 are essential for promoter function. The DNA sequences upstream from -374 modulate the level of expression in leaf tissue; this modulation is under developmental control.
摘要
通过农杆菌介导的转化，我们已经证明，来自番茄rbcS - 3A基因的1.10千碱基启动子序列与细菌氯霉素乙酰转移酶基因融合后，可赋予光诱导性和器官特异性表达。通过对该启动子进行5'缺失分析，获得了双相表达谱，表明存在正调控元件和负调控元件。当从1.10千碱基启动子中缺失5'末端的90个碱基对时，观察到表达水平严重降低。负责该基因光诱导性和器官特异性的DNA序列元件位于启动子近端的-374碱基对内，从-374到-205的序列对于启动子功能至关重要。-374上游的DNA序列调节叶片组织中的表达水平；这种调节受发育控制。
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