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A simple and general method for transferring genes into plants.一种将基因转入植物的简单而通用的方法。
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Localization in the Tomato Genome of DNA Restriction Fragments Containing Sequences Homologous to the rRNA (45s), the Major Chlorophyll a/b Binding Polypeptide and the Ribulose Bisphosphate Carboxylase Genes.在番茄基因组中，DNA 限制片段的定位包含与 rRNA（45s）、主要叶绿素 a/b 结合多肽和核酮糖二磷酸羧化酶基因同源的序列。
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COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.分离叶绿体中的铜酶。甜菜中的多酚氧化酶。
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Developmental regulation of two genes encoding ribulose-bisphosphate carboxylase small subunit in pea and transgenic petunia plants: Phytochrome response and blue-light induction.豌豆和转基因矮牵牛植物中编码核酮糖二磷酸羧化酶小亚基的两个基因的发育调控：光敏色素反应和蓝光诱导。
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Cloning, mapping, and in vitro transcription-translation of the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase from spinach chloroplasts.菠菜叶绿体 RuBP 羧化酶大亚基基因的克隆、定位和体外转录-翻译。
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5' Analysis of the soybean leghaemoglobin lbc(3) gene: regulatory elements required for promoter activity and organ specificity.大豆血红蛋白 lbc(3)基因的 5' 分析：启动子活性和器官特异性所需的调控元件。
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Photoregulated expression of a pea rbcS gene in leaves of transgenic plants.光调控豌豆 rbcS 基因在转基因植物叶片中的表达。
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Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity.Ti 质粒载体可将 DNA 导入植物细胞而不改变其正常的再生能力。
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番茄rbcS - 3A基因的表达水平受一个远上游启动子元件以发育调控的方式调节。

Level of expression of the tomato rbcS-3A gene is modulated by a far upstream promoter element in a developmentally regulated manner.

作者信息

Ueda T, Pichersky E, Malik V S, Cashmore A R

机构信息

Department of Biology, University of Pennsylvania, Philadelphia 19104-6018.

出版信息

Plant Cell. 1989 Feb;1(2):217-27. doi: 10.1105/tpc.1.2.217.

DOI:10.1105/tpc.1.2.217
PMID:2535544
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC159754/
Abstract

By Agrobacterium-mediated transformation we have demonstrated that a 1.10-kilobase promoter sequence from the tomato rbcS-3A gene confers light-inducible and organ-specific expression upon fusion to the bacterial chloramphenicol acetyltransferase gene. A biphasic expression profile was obtained by 5' deletion analysis of this promoter, indicating the presence of both positive and negative regulatory elements. A severe reduction in the level of expression was observed when the 5'-terminal 90 base pairs were deleted from the 1.10-kilobase promoter. DNA sequence elements responsible for light inducibility and organ specificity of the gene reside within the -374 base pairs of the proximal part of the promoter and the sequences spanning from -374 to -205 are essential for promoter function. The DNA sequences upstream from -374 modulate the level of expression in leaf tissue; this modulation is under developmental control.

摘要

通过农杆菌介导的转化，我们已经证明，来自番茄rbcS - 3A基因的1.10千碱基启动子序列与细菌氯霉素乙酰转移酶基因融合后，可赋予光诱导性和器官特异性表达。通过对该启动子进行5'缺失分析，获得了双相表达谱，表明存在正调控元件和负调控元件。当从1.10千碱基启动子中缺失5'末端的90个碱基对时，观察到表达水平严重降低。负责该基因光诱导性和器官特异性的DNA序列元件位于启动子近端的-374碱基对内，从-374到-205的序列对于启动子功能至关重要。-374上游的DNA序列调节叶片组织中的表达水平；这种调节受发育控制。