磁场暴露对小鼠的睾丸毒性作用以及辅酶Q10和左旋肉碱的预防作用
Testicular toxicity effects of magnetic field exposure and prophylactic role of coenzyme Q10 and L-carnitine in mice.
作者信息
Ramadan Laila A, Abd-Allah Adel R A, Aly Hamdy A A, Saad-el-Din Aisha A
机构信息
Drug Radiation Research Department, NCRRT, Atomic Energy Authority, P.O. Box 29, Nasr City, Cairo, Egypt.
出版信息
Pharmacol Res. 2002 Oct;46(4):363-70. doi: 10.1016/s1043661802001718.
The protective effect of L-carnitine or coenzyme Q10 (CoQ10) against high magnetic field (20 mT) induced testicular toxicity in mice were evaluated. Animals were injected with L-carnitine (200 mg kg(-1), i.p.) or CoQ10 (200 mg kg(-1), p.o.) 1h before exposure to fractionated doses (30 min per day, three times per week for 2 weeks) or acute dose (3h) of magnetic field. Total sperm count, motility, daily sperm production, and testicular LDH-X activity as well as histopathological examination were investigated. Exposure of mice to fractionated doses of magnetic field caused a significant decrease in sperm count, motility, daily sperm production, and LDH-X activity, which was more pronounced than that of acute dose. Moreover, a marked testicular histopathological changes were observed after exposure to fractionated doses of magnetic field. Pretreatment of mice with L-carnitine or CoQ10 1h before exposure to magnetic field caused a significant recovery of mice testes damage induced by high magnetic field (20 mT).
评估了左旋肉碱或辅酶Q10（CoQ10）对高磁场（20 mT）诱导的小鼠睾丸毒性的保护作用。在暴露于分次剂量（每天30分钟，每周三次，共2周）或急性剂量（3小时）的磁场前1小时，给动物注射左旋肉碱（200 mg kg(-1)，腹腔注射）或CoQ10（200 mg kg(-1)，口服）。研究了精子总数、活力、每日精子生成量、睾丸乳酸脱氢酶-X（LDH-X）活性以及组织病理学检查。小鼠暴露于分次剂量的磁场会导致精子数量、活力、每日精子生成量和LDH-X活性显著下降，这比分次剂量更为明显。此外,在暴露于分次剂量的磁场后观察到明显的睾丸组织病理学变化。在暴露于磁场前1小时用左旋肉碱或CoQ10预处理小鼠，可显著恢复高磁场（20 mT）诱导的小鼠睾丸损伤。
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