Karimi Fatemeh, Khodabandeh Zahra, Nazari Fatemeh, Dara Mahintaj, Masjedi Fatemeh, Momeni-Moghaddam Madjid
Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Reprod Sci. 2023 Oct;30(10):2962-2972. doi: 10.1007/s43032-023-01240-w. Epub 2023 Apr 18.
Studies on adverse health consequences of azo dyes are limited and conflicting. Coenzyme Q10 (CoQ10) supplementation has been shown to have benefits associated with antioxidant and anti-inflammatory characteristics on several body systems. This work investigates the possible toxic effects of the widely used food additive sunset yellow and the probable protective effects of CoQ10 on testicular tight and gap junctions in rats by assessing molecular, immunohistochemical, and histopathological changes. Sixty Sprague-Dawley male weanling rats were randomly divided into six groups (n = 10). The rats received their treatments via daily oral gavages for 6 weeks. The treatments included as follows: low dose of sunset yellow (SY-LD) (2.5 mg/kg/day), high dose of sunset yellow (SY-HD) (70 mg/kg/day), CoQ10 (10 mg/kg/day), CoQ10 with low dose of sunset yellow (CoQ10 + LD), CoQ10 with high dose of sunset yellow (CoQ10 + HD), and distilled water as the control treatment. At the end of the experiment, the rats were anesthetized, and the testes were removed for molecular (real-time quantitative PCR), immunohistochemical, and histopathological (H & E staining) assessments. Claudin 11 and occludin gene expression significantly decreased in HD and CoQ10 + HD groups compared with the controls. Connexin 43 (Cx43) expression in the control and CoQ10 groups was significantly higher than in the HD group. The immunohistochemical and histopathological data were largely in line with these findings. The results showed that exposure to a high dose of sunset yellow led to disturbances in cell-to-cell interactions and testicular function. Simultaneous treatment with CoQ10 had some beneficial effects but did not completely improve these undesirable effects.
关于偶氮染料对健康的不良影响的研究有限且相互矛盾。补充辅酶Q10（CoQ10）已被证明对多个身体系统具有与抗氧化和抗炎特性相关的益处。这项工作通过评估分子、免疫组织化学和组织病理学变化，研究了广泛使用的食品添加剂日落黄可能的毒性作用以及CoQ10对大鼠睾丸紧密连接和缝隙连接的潜在保护作用。将60只Sprague-Dawley雄性断奶大鼠随机分为六组（n = 10）。大鼠通过每日口服灌胃接受治疗，持续6周。治疗包括以下几种：低剂量日落黄（SY-LD）（2.5毫克/千克/天）、高剂量日落黄（SY-HD）（70毫克/千克/天）、CoQ10（10毫克/千克/天）、CoQ10与低剂量日落黄（CoQ10 + LD）、CoQ10与高剂量日落黄（CoQ10 + HD），以及蒸馏水作为对照治疗。实验结束时，将大鼠麻醉，取出睾丸进行分子（实时定量PCR）、免疫组织化学和组织病理学（苏木精和伊红染色）评估。与对照组相比，HD组和CoQ10 + HD组中Claudin 11和闭合蛋白基因表达显著降低。对照组和CoQ10组中连接蛋白43（Cx43）的表达显著高于HD组。免疫组织化学和组织病理学数据在很大程度上与这些发现一致。结果表明，高剂量暴露于日落黄会导致细胞间相互作用和睾丸功能紊乱。同时用CoQ10治疗有一些有益效果，但并未完全改善这些不良影响。
