神经再生的纳米医学工程：发光二极管介导的超顺磁性氧化铁-金核壳纳米粒子，通过神经生长因子功能化。
Engineered nanomedicine for neuroregeneration: light emitting diode-mediated superparamagnetic iron oxide-gold core-shell nanoparticles functionalized by nerve growth factor.
机构信息
J. Mike Walker '66 Department of Mechanical Engineering, Texas A&M University, College Station, TX.
Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY.
出版信息
Nanomedicine. 2019 Oct;21:102052. doi: 10.1016/j.nano.2019.102052. Epub 2019 Jul 23.
Abstract
This paper reports nerve growth factor functionalized superparamagnetic iron oxide-gold core-shell nanoparticles (NGF-SPIO-Au NPs), an engineered nanomedicine for non-invasive neuron regeneration when irradiated by a low-intensity light-emitting diode (LED). NGF-SPIO-Au NPs of 20 μg/ml, were tested on PC-12 neuron-like cells, irradiated by LEDs (525 nm, 1.09, 1.44, and 1.90 mW/cm). A remarkable Ca influx was detected in differentiated PC-12 cells treated with NPs, irradiated by LED of 1.90 and 1.44 mW/cm with great cell viability (>84%) and proliferations. The strong heat generated through their plasmonic surface upon LED irradiation on NGF-SPIO-Au NPs was observed. For cells treated with LED (1.90 mW/cm) and NGF-SPIO-Au NPs, a dramatic enhancement of neuronal differentiation (83%) and neurite outgrowth (51%) was found, and the upregulation of both the neural differentiation specific marker (β3-tubulin) and the cell adhesive molecule (integrin β1) was observed by the reverse transcription-polymerase chain reaction and western blot analysis.
摘要
本文报道了神经生长因子功能化超顺磁性氧化铁-金核壳纳米粒子（NGF-SPIO-Au NPs），这是一种工程纳米医学药物，可在低强度发光二极管（LED）照射下进行非侵入性神经元再生。将 20 μg/ml 的 NGF-SPIO-Au NPs 用于经 LED（525nm、1.09、1.44 和 1.90mW/cm）照射的 PC-12 神经元样细胞。在经 1.90 和 1.44mW/cm LED 照射并用 NPs 处理的分化 PC-12 细胞中检测到明显的 Ca 内流，细胞活力（>84%）和增殖率非常高。在 NGF-SPIO-Au NPs 上的等离子体表面通过 LED 照射产生了强烈的热量。对于用 LED（1.90mW/cm）和 NGF-SPIO-Au NPs 处理的细胞，发现神经元分化（83%）和神经突生长（51%）显著增强，并且通过逆转录-聚合酶链反应和 Western blot 分析观察到神经分化特异性标记物（β3-微管蛋白）和细胞黏附分子（整合素β1）的上调。
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