Kang Jeong-Hun, Kondo Fusao
Department of Veterinary Public Health, Faculty of Agriculture, Miyazaki University, Gakuen, Japan.
Chemosphere. 2002 Nov;49(5):493-8. doi: 10.1016/s0045-6535(02)00315-6.
Total 15 surface river waters were collected from thirteen different rivers to investigate a relationship of bacterial counts and temperature to the degradation of bisphenol A (BPA). Autoclaved and non-autoclaved river water samples were spiked with 0.2 mg/l BPA. The spiked samples were placed at temperatures of 4, 20, and 30 degrees C and analyzed by high performance liquid chromatography. BPA was degraded at all temperatures in the non-autoclaved samples. However, BPA in the autoclaved samples was not changed at all temperatures for 20 d. These results show that the primary factor of BPA degradation in river water is bacteria. Moreover, three groups [group A (> 10000 CFU/ml), group B (2000-10000 CFU/ml), and group C (< 2000 CFU/ml)], were made on the basis of bacterial counts of the samples. Half-lives for BPA degradation in groups A, B, and C were 2, 3, and 6 d at 30 degrees C and were 4, 5, and 7 d at 20 degrees C, respectively. But at 4 degrees C, the loss of BPA was about 40%, 20%, and 10% in groups A, B, and C for 20 d, respectively. Bacterial counts exerted an influence on BPA degradation in river water with temperature. Our results also show that BPA-degrading bacteria are widely distributed in river waters.
从13条不同的河流中总共采集了15份地表水样本，以研究细菌数量和温度与双酚A（BPA）降解之间的关系。向经过高压灭菌和未经过高压灭菌的河水样本中添加0.2 mg/l的BPA。将添加后的样本置于4、20和30摄氏度的温度下，并通过高效液相色谱法进行分析。在未经过高压灭菌的样本中，BPA在所有温度下均发生降解。然而，经过高压灭菌的样本中的BPA在所有温度下20天内均未发生变化。这些结果表明，河水中BPA降解的主要因素是细菌。此外，根据样本的细菌数量分为三组[A组（> 10000 CFU/ml）、B组（2000 - 10000 CFU/ml）和C组（< 2000 CFU/ml）]。在30摄氏度时，A组、B组和C组中BPA降解的半衰期分别为2天、3天和6天；在20摄氏度时，分别为4天、5天和7天。但在4摄氏度时，20天内A组、B组和C组中BPA的损失分别约为40%、20%和10%。细菌数量对不同温度下河水中BPA的降解有影响。我们的结果还表明，降解BPA的细菌广泛分布于河水中。
