Kang Jeong-Hun, Kondo Fusao
Department of Applied Chemistry, Faculty of Engineering, Kyushu University, 6-10-1 Hakozaki, Higashi-Ku, Fukuoka 812-8581, Japan.
Chemosphere. 2005 Sep;60(9):1288-92. doi: 10.1016/j.chemosphere.2005.01.058. Epub 2005 Mar 2.
The purpose of this study was to identify a relationship between changes of bacterial counts and bisphenol A (BPA) degradation in seawater under aerobic or anaerobic conditions, and at temperatures of 4, 25, and 35 degrees C. Water samples (seawater and river water) spiked with 1000 ng/ml of BPA was placed for 60 d. The BPA from water samples was extracted by OASIS HLB cartridges and was detected by high-performance liquid chromatography with fluorescence detection. BPA in river water was degraded under aerobic conditions and was below a detection limit (0.5 ng/ml) on the seventh day at both 25 and 35 degrees C. The more the level of bacterial counts increased, the more BPA degradation decreased. In the case of seawater samples, there was no relationship between BPA degradation and the change of bacterial counts. Bacterial counts at 25 and 35 degrees C increased rapidly at 5 and 3d, respectively, but decreased since then. The concentration of BPA was not changed for 30 d at both 25 and 35 degrees C, but decreased from 40 to 60 d in spite of low levels of bacteria. These results show that the different degradation way for BPA in seawater may exist. Moreover, our study suggest that BPA in seawater than in river water can continue for longer time with no degradation and the possibility of BPA contamination on a marine organism can be higher than that on freshwater organism.
本研究的目的是确定在有氧或无氧条件下，以及在4℃、25℃和35℃的温度下，海水中细菌数量的变化与双酚A（BPA）降解之间的关系。将添加了1000 ng/ml BPA的水样（海水和河水）放置60天。水样中的BPA通过OASIS HLB柱进行萃取，并通过带荧光检测的高效液相色谱法进行检测。河水中的BPA在有氧条件下降解，在25℃和35℃时，第7天均低于检测限（0.5 ng/ml）。细菌数量增加得越多，BPA降解得越少。对于海水样品，BPA降解与细菌数量变化之间没有关系。25℃和35℃时的细菌数量分别在第5天和第3天迅速增加，但此后下降。在25℃和35℃时，BPA浓度在30天内没有变化，但尽管细菌数量较少，在40至60天时BPA浓度下降。这些结果表明，海水中可能存在不同的BPA降解方式。此外，我们的研究表明，海水中的BPA比河水中的BPA能够在更长时间内不降解，并且BPA对海洋生物造成污染的可能性可能高于对淡水生物的污染。
