Bell A S, Sommerville C
Institute of Aquaculture, University of Stirling, Stirling, FK9 4LA, UK.
J Helminthol. 2002 Sep;76(3):193-8. doi: 10.1079/JOH2002120.
The current study examined rDNA (internal transcribed spacer regions, ITS1 and ITS2) and cytochrome c oxidase subunit 1 (CO1) sequence data of Apatemon annuligerum (originating from two geographical locations) and A. gracilis metacercariae (originating from four natural piscine hosts) to determine the systematic status of these two strigeid digeneans. With the exception of short repeat motifs, the ITS1 regions sequenced demonstrated no intra- or inter-specific sequence variation. ITS2 sequences were 292 bp and CO1 sequences 366 bp in length and identical for both nominal Apatemon species. These sequence data provide strong evidence that the two species are con-specific and that A. annuligerum should be regarded as a junior synonym of A. gracilis.
本研究检测了环形阿帕特吸虫（来自两个地理位置）和纤细阿帕特吸虫后尾蚴（来自四种天然鱼类宿主）的核糖体DNA（内转录间隔区，ITS1和ITS2）以及细胞色素c氧化酶亚基1（CO1）序列数据，以确定这两种斜睾科复殖吸虫的系统地位。除短重复基序外，所测序的ITS1区域未显示种内或种间序列变异。ITS2序列长度为292 bp，CO1序列长度为366 bp，两种名义上的阿帕特吸虫物种的这些序列相同。这些序列数据提供了有力证据，表明这两个物种为同种，环形阿帕特吸虫应被视为纤细阿帕特吸虫的次异名。
