Senapin S, Phiwsaiya K, Laosinchai P, Kowasupat C, Ruenwongsa P, Panijpan B
National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani, 12120, Thailand;
J Parasitol. 2014 Jun;100(3):368-71. doi: 10.1645/13-285.1. Epub 2014 Feb 3.
Many species of fish in the world are infected with digenean trematodes belonging to the genera Clinostomum and Euclinostomum. In this study, metacercariae, identified as Euclinostomum sp. on the basis of morphological characteristics and molecular data, were taken from 3 osphronemid fish- Trichopsis vittata , Trichopsis schalleri, and Betta imbellis, in Thailand. Phylogenetic analysis based on a mitochondrial gene (cytochrome c oxidase subunit I) and 2 nuclear genes (18S rDNA and ITS-internal transcribed spacer) of these Euclinostomum parasites indicated a clear distinction from those belonging to the Clinostomum genus. These are the first records of partial mitochondrial and nuclear DNA sequences of an Euclinostomum sp.
世界上许多鱼类都感染了属于斜睾属和真斜睾属的复殖吸虫。在本研究中，根据形态特征和分子数据鉴定为真斜睾属物种的后尾蚴，取自泰国的3种斗鱼——五线斗鱼、施氏斗鱼和钝身斗鱼。基于这些真斜睾属寄生虫的线粒体基因（细胞色素c氧化酶亚基I）和2个核基因（18S rDNA和ITS-内部转录间隔区）的系统发育分析表明，它们与斜睾属的寄生虫有明显区别。这些是真斜睾属物种部分线粒体和核DNA序列的首次记录。
