整合宿主因子（IHF）和组蛋白样蛋白（HU）通过两种不同机制刺激大肠杆菌oriC处前复制复合物的组装。
IHF and HU stimulate assembly of pre-replication complexes at Escherichia coli oriC by two different mechanisms.
作者信息
Ryan Valorie T, Grimwade Julia E, Nievera Christian J, Leonard Alan C
机构信息
Department of Biological Services, Florida Institute of Technology, 150 W. University Blvd, Melbourne, Florida 32901, USA.
出版信息
Mol Microbiol. 2002 Oct;46(1):113-24. doi: 10.1046/j.1365-2958.2002.03129.x.
Pre-replication complexes (pre-RC) assemble on replication origins and unwind DNA in the presence of chromatin proteins. As components of Escherichia coli pre-RC, two histone-like proteins HU and IHF (integration host factor), stimulate initiator DnaA-catalysed unwinding of the chromosomal replication origin, oriC. Using in vivo footprint analysis just before DNA synthesis initiates, we detect IHF binding coincident with a shift of DnaA to weaker central oriC sites. Integration host factor redistributed pre-bound DnaA to identical sites in vitro. HU did not redistribute DnaA, but suppressed binding specifically at I3. These results suggest that different pathways mediated by bacterial chromatin proteins exist to regulate pre-RC assembly and unwind oriC.
复制前复合体（pre-RC）在复制起点组装，并在染色质蛋白存在的情况下解开DNA。作为大肠杆菌pre-RC的组成部分，两种组蛋白样蛋白HU和整合宿主因子（IHF）刺激引发剂DnaA催化的染色体复制起点oriC的解旋。在DNA合成开始前使用体内足迹分析，我们检测到IHF结合与DnaA向较弱的中央oriC位点的转移同时发生。整合宿主因子在体外将预先结合的DnaA重新分布到相同位点。HU没有重新分布DnaA，但特异性抑制I3位点的结合。这些结果表明，细菌染色质蛋白介导的不同途径存在以调节pre-RC组装和解开oriC。
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