基于基因序列比较分析对犬血巴尔通体和溶血支原体进行鉴别
Differentiation of Haemobartonella canis and Mycoplasma haemofelis on the basis of comparative analysis of gene sequences.
作者信息
Birkenheuer Adam J, Breitschwerdt Edward B, Alleman A Rick, Pitulle Christian
机构信息
Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh 27606-1428, USA.
出版信息
Am J Vet Res. 2002 Oct;63(10):1385-8. doi: 10.2460/ajvr.2002.63.1385.
OBJECTIVE
To determine whether Haemobartonella canis and Mycoplasma haemofelis (formerly known as H felis [large form]) can be differentiated by use of comparative analysis of gene sequences.
SAMPLE POPULATION
Blood samples obtained from 3 dogs infected with H canis and 2 cats infected with M haemofelis.
PROCEDURE
The partial 16S rDNA and ribonuclease P RNA (RNase P) genes were amplified, cloned, and sequenced in blood samples obtained from H canis-infected dogs and M haemofelis-infected cats. The DNA sequences were subjected to comparative analysis.
RESULTS
The 16S rDNA sequences of H canis and M haemofelis were nearly identical (homology of 99.3 to 99.7%). In contrast, RNase P gene sequences had a lower degree of sequence homology between the 2 organisms (94.3 to 95.5%).
CONCLUSIONS AND CLINICAL RELEVANCE
Haemobartonella canis and M haemofelis are not identical organisms. Molecular differentiation of H canis and M haemofelis is more clearly evident by use of comparative analysis of RNase P gene sequences than by comparative analysis of 16S rDNA gene sequences.
目的
通过基因序列比较分析来确定犬血巴尔通体和溶血支原体（以前称为溶血巴尔通体[大型形态]）是否可以区分。
样本群体
从3只感染犬血巴尔通体的犬和2只感染溶血支原体的猫获取血液样本。
步骤
对从感染犬血巴尔通体的犬和感染溶血支原体的猫获取的血液样本中的部分16S rDNA和核糖核酸酶P RNA（RNase P）基因进行扩增、克隆和测序。对DNA序列进行比较分析。
结果
犬血巴尔通体和溶血支原体的16S rDNA序列几乎相同（同源性为99.3%至99.7%）。相比之下，两种生物体之间的RNase P基因序列具有较低程度的序列同源性（94.3%至95.5%）。
结论及临床意义
犬血巴尔通体和溶血支原体不是相同的生物体。通过对RNase P基因序列进行比较分析比通过对16S rDNA基因序列进行比较分析，犬血巴尔通体和溶血支原体的分子区分更为明显。
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