Waterman-Storer Clare M, Danuser Gaudenz
Department of Cell Biology and Institute for Childhood and Neglected Diseases, The Scripps Research Institute, La Jolla, CA 92037, USA.
Curr Biol. 2002 Sep 17;12(18):R633-40. doi: 10.1016/s0960-9822(02)01139-9.
Fluorescent Speckle Microscopy (FSM) is a technology for analyzing cytoskeleton dynamics, giving novel insight into their roles in living cells. New applications of FSM, together with the development of computer-based FSM image analysis, will make FSM the first microscopy-based method to deliver quantitative kinetic readouts at high spatial and temporal resolution for a wide variety of macromolecular systems. Here, we review the most recent applications and developments and give a glimpse of future directions and potentials of FSM.
荧光斑点显微镜技术（FSM）是一种用于分析细胞骨架动力学的技术，能为其在活细胞中的作用提供全新见解。FSM的新应用，以及基于计算机的FSM图像分析技术的发展，将使FSM成为第一种基于显微镜的方法，能够在高空间和时间分辨率下为多种大分子系统提供定量动力学读数。在此，我们回顾了FSM的最新应用和发展情况，并展望了其未来的方向和潜力。
