Tegnell A, Saeedi B, Isaksson B, Granfeldt H, Ohman L
Division of Infectious Diseases, Department of Health and Environment, Linköping University, Sweden.
J Hosp Infect. 2002 Sep;52(1):37-42. doi: 10.1053/jhin.2002.1267.
Coagulase-negative staphylococci (CoNS) are important causes of hospital-acquired infections such as infections after cardiac surgery. Efforts to reduce these infections are hampered by the lack of knowledge concerning the epidemiology of CoNS in this setting. Forty strains of CoNS collected during the surgical revision of 27 patients operated on between 1997 and 2000 were analysed. Strains were also collected from the ambient air in the operating suite. Their pulsed-field gel electrophoresis (PFGE) characteristics and antibiotic resistance were analysed. Using PFGE 19 of 40 strains from 15 of 27 patients were shown to belong to one clone, and strains from this clone were also isolated from the ambient air. This clone had caused infections throughout the period. Antibiotic resistance did not correlate with PFGE patterns. Using PFGE one clone could be identified that caused 56% of the CoNS infections during this period. A strain from this clone was also found in the air of the operating suite suggesting the origin of the CoNS causing infections was the hospital environment.
凝固酶阴性葡萄球菌（CoNS）是医院获得性感染的重要病因，如心脏手术后感染。由于缺乏关于这种情况下CoNS流行病学的知识，减少这些感染的努力受到阻碍。对1997年至2000年间接受手术的27例患者在手术翻修期间收集的40株CoNS进行了分析。还从手术室的环境空气中收集了菌株。分析了它们的脉冲场凝胶电泳（PFGE）特征和抗生素耐药性。使用PFGE分析发现，27例患者中的15例的40株菌株中有19株属于一个克隆，并且该克隆的菌株也从环境空气中分离出来。该克隆在整个时期都引起了感染。抗生素耐药性与PFGE模式无关。使用PFGE可以鉴定出一个克隆，该克隆在此期间引起了56%的CoNS感染。在手术室的空气中也发现了来自该克隆的一株菌株，这表明引起感染的CoNS的来源是医院环境。
