van Belkum A, Kluijtmans J, van Leeuwen W, Goessens W, ter Averst E, Verbrugh H
Department of Bacteriology, University Hospital Dijkzigt, Rotterdam, Netherlands.
J Hosp Infect. 1995 Dec;31(4):285-93. doi: 10.1016/0195-6701(95)90207-4.
Forty-six strains of coagulase-negative staphylococci (CNS) were typed by biochemical reactivity, antibiotic susceptibility pattern, macrorestriction analysis by pulsed-field gel electrophoresis (PFGE) and arbitrary primed polymerase chain reaction (AP PCR). Twenty-four strains were obtained in 1993 from blood cultures of as many patients coupled to a heart-lung machine during cardiac surgery. Since over 30% of the latter belonged to a single type, it was concluded that during the year of analysis a single clone of CNS persisted in this hospital setting. Subsequent epidemiological surveillance putatively identified four possible carriers among surgical personnel. For this reason, 22 strains collected from the hands and nose of two cardiac surgeons and two perfusionists were also tested; none were identical to the persistent clone. Thus either the operation equipment was colonized longitudinally or the causative CNS had disappeared from the suspect individuals' flora. Longitudinal monitoring of CNS infections by various techniques gives a valuable insight into nosocomial epidemiology and elucidates the complexity of the CNS colonization.
通过生化反应性、抗生素敏感性模式、脉冲场凝胶电泳（PFGE）进行的宏观限制性分析以及任意引物聚合酶链反应（AP PCR）对46株凝固酶阴性葡萄球菌（CNS）进行了分型。1993年从心脏手术期间与心肺机相连的同样多患者的血培养物中获得了24株菌株。由于后者中超过30%属于单一类型，得出结论，在分析年份期间，一种单一的CNS克隆在该医院环境中持续存在。随后的流行病学监测推测在手术人员中确定了四名可能的携带者。因此，还对从两名心脏外科医生和两名灌注师的手和鼻中采集的22株菌株进行了检测；没有一株与持续存在的克隆相同。因此，要么手术设备被纵向定植，要么致病性CNS已从可疑个体的菌群中消失。通过各种技术对CNS感染进行纵向监测，为医院感染流行病学提供了有价值的见解，并阐明了CNS定植的复杂性。
