Witz Craig A, Cho Sook, Montoya-Rodriguez Iris A, Schenken Robert S
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, University of Texas Health Science Center at San Antonio, Texas, San Antonio 78229-3900, USA.
Fertil Steril. 2002 Oct;78(4):796-803. doi: 10.1016/s0015-0282(02)03340-x.
To evaluate the possible role of mesothelial alpha(2)beta(1) and alpha(3)beta(1) integrins in the attachment of endometrial stromal cells (ESCs) and endometrial epithelial cells (EECs).
In vitro study.
University medical center.
PATIENT(S): Women of reproductive age (n = 26).
MAIN OUTCOME MEASURE(S): Mesothelial cells were grown on collagen IV. Endometrial stromal cells and EECs were plated on mesothelial cells for 1 hour. Before plating, mesothelial cells or endometrial cells were incubated with antibodies to alpha2, alpha3, and beta1 integrin subunits. The effect of these antibodies on ESC and EEC binding to collagen IV and collagen I was also examined. The expression of collagen I, collagen IV, fibronectin, and laminin by cultured ESCs and EECs was examined.
RESULT(S): The anti-integrin antibodies had no effect on endometrial binding to mesothelium. The beta1 integrin antibody decreased binding of ESCs and EECs to the collagen matrices. In culture, ESCs and EECs expressed collagen I, collagen IV, fibronectin, and laminin to varying degrees.
CONCLUSION(S): The initial adhesion of ESCs and EECs to mesothelium is not mediated by beta1 integrins. In contrast, ESC and EEC attachment to collagen IV and collagen I, which are present in the submesothelial extracellular matrix, is mediated by beta1 integrins.
评估间皮α(2)β(1)和α(3)β(1)整合素在子宫内膜基质细胞（ESC）和子宫内膜上皮细胞（EEC）黏附中的可能作用。
体外研究。
大学医学中心。
育龄期女性（n = 26）。
间皮细胞在IV型胶原上生长。将子宫内膜基质细胞和EEC铺在间皮细胞上1小时。铺板前，将间皮细胞或子宫内膜细胞与α2、α3和β1整合素亚基的抗体孵育。还检测了这些抗体对ESC和EEC与IV型胶原和I型胶原结合的影响。检测培养的ESC和EEC中I型胶原、IV型胶原、纤连蛋白和层粘连蛋白的表达。
抗整合素抗体对子宫内膜与间皮的黏附无影响。β1整合素抗体降低了ESC和EEC与胶原基质的结合。在培养中，ESC和EEC不同程度地表达I型胶原、IV型胶原、纤连蛋白和层粘连蛋白。
ESC和EEC与间皮的初始黏附不是由β1整合素介导的。相反，ESC和EEC与间皮下细胞外基质中存在的IV型胶原和I型胶原的黏附是由β1整合素介导的。
