Chen Bingzi, Jamieson Elizabeth R, Tullius Thomas D
Department of Chemistry, Boston University, Boston, MA 02215, USA.
Bioorg Med Chem Lett. 2002 Nov 4;12(21):3093-6. doi: 10.1016/s0960-894x(02)00650-9.
An efficient procedure is described for the preparation of ribonucleotides and deoxyribonucleotides with deuterium incorporated at the 1', 4', or 5' position. Three intermediates-[1-2H]-D-ribose, [4-2H]-D-ribose, and [5-2H(2)]-D-ribose-were prepared by chemical synthesis and subsequently converted to ribonucleotides and deoxyribonucleotides via enzymatic reactions. Milligram quantities of the desired products were obtained with an average deuterium content of 96+/-1%.
本文描述了一种高效的制备方法，用于制备在1'、4'或5'位置掺入氘的核糖核苷酸和脱氧核糖核苷酸。通过化学合成制备了三种中间体——[1-²H]-D-核糖、[4-²H]-D-核糖和[5-²H₂]-D-核糖，随后通过酶促反应将它们转化为核糖核苷酸和脱氧核糖核苷酸。获得了毫克量的所需产物，其平均氘含量为96±1%。
