Sim Soyeong, Kim Kwang-sun, Lee Younghoon
Department of Chemistry, Center for Molecular Design and Synthesis, Korea Advanced Institute of Science and Technology, Taejon 305-701, South Korea.
FEBS Lett. 2002 Oct 9;529(2-3):225-31. doi: 10.1016/s0014-5793(02)03345-8.
M1 RNA, the catalytic component of Escherichia coli RNase P, is derived from the 3'-end processing of precursor M1 RNA, a major transcript of the rnpB gene. In this study, we investigated the mechanism of 3'-end processing of M1 RNA using the recombinant N-terminal half RNase E. The cleavage site preference of RNase E differed from that of the 40% ammonium sulfate precipitate (ASP-40), a partially purified cell extract containing processing activity. However, the addition of a trace amount of ASP-40 changed the cleavage site preference of RNase E to that of ASP-40 suggesting the involvement of a soluble factor in cleavage site preference.
M1 RNA是大肠杆菌核糖核酸酶P的催化成分，它来源于前体M1 RNA的3'端加工，前体M1 RNA是rnpB基因的主要转录产物。在本研究中，我们使用重组N端半核糖核酸酶E研究了M1 RNA的3'端加工机制。核糖核酸酶E的切割位点偏好与40%硫酸铵沉淀（ASP - 40）不同，ASP - 40是一种含有加工活性的部分纯化细胞提取物。然而，添加微量的ASP - 40会使核糖核酸酶E的切割位点偏好变为ASP - 40的偏好，这表明一种可溶性因子参与了切割位点偏好的形成。
