Rasmussen E M, Sørensen E, Eriksen B, Larsen H J, Morling N
Department of Forensic Genetics, Institute of Forensic Medicine, University of Copenhagen, Frederik V's Vej 11, DK-2100 Copenhagen, Denmark.
Forensic Sci Int. 2002 Oct 9;129(3):209-13. doi: 10.1016/s0379-0738(02)00276-1.
We describe a method to obtain reliable mitochondrial DNA (mtDNA) sequences downstream of the homopolymeric stretches with length heteroplasmy in the sequencing direction. The method is based on the use of junction primers that bind to a part of the homopolymeric stretch and the first 2-4 bases downstream of the homopolymeric region. This junction primer method gave clear and unambiguous results using samples from 21 individuals with length heteroplasmy in the hypervariable regions HV1, HV2 or both. The method is of special value for forensic casework, because sequencing of both strands of an mtDNA region is preferable in order to reduce ambiguities in sequence determination.
我们描述了一种方法，用于在测序方向上获得具有长度异质性的同聚物延伸下游可靠的线粒体DNA（mtDNA）序列。该方法基于使用连接引物，该引物结合到同聚物延伸的一部分以及同聚物区域下游的前2 - 4个碱基。使用来自21名在高变区HV1、HV2或两者中具有长度异质性的个体的样本，这种连接引物方法给出了清晰明确的结果。该方法对于法医案件工作具有特殊价值，因为为了减少序列测定中的歧义，mtDNA区域的两条链进行测序是更可取的。
