Jarry Claire, Leroux Jean-Christophe, Haeck Jonathan, Chaput Cyril
Canada Research Chair in Drug Delivery, Faculty of Pharmacy, University of Montreal.
Chem Pharm Bull (Tokyo). 2002 Oct;50(10):1335-40. doi: 10.1248/cpb.50.1335.
The effects of steam sterilization and gamma-irradiation on chitosan and thermogelling chitosan-beta-glycerophosphate (GP) solutions containing polyol additives were investigated. The selected polyols were triethylene glycol, glycerol, sorbitol, glucose and poly(ethylene glycol) (PEG). They were incorporated to chitosan solutions prior to sterilization in a proportion ranging from 1 to 5% (w/v). The solutions were characterized with respect to their viscosity, thermogelling properties, compressive stress relaxation behavior and chitosan degradation. All polyols reduced the autoclaving-induced viscosity loss and had a positive impact on the solution thermogelling properties and compressive performance of the gels. Steam sterilization in the presence of glucose resulted in a substantial increase in the solution viscosity and gel strength. This was associated with a strong discoloration suggesting chemical alteration of the system. PEG was the most effective agent in preventing hydrolytic degradation of chitosan chains. Gamma-irradiation strongly decreased the chitosan solution viscosity regardless of the presence of additives, even when sterilization was carried out at -80 degrees C. Moreover, the thermogelling properties were dramatically altered, and thus, gamma-irradiation would not be an appropriate method to sterilize chitosan solutions. In conclusion, polyols are potentially useful additive to maximise the viscoelastic and mechanical properties of chitosan-GP after steam sterilization.
研究了蒸汽灭菌和γ射线辐照对含有多元醇添加剂的壳聚糖以及热凝胶化壳聚糖-β-甘油磷酸酯（GP）溶液的影响。所选的多元醇为三甘醇、甘油、山梨醇、葡萄糖和聚乙二醇（PEG）。在灭菌前，它们以1%至5%（w/v）的比例加入到壳聚糖溶液中。对溶液的粘度、热凝胶化性质、压缩应力松弛行为和壳聚糖降解进行了表征。所有多元醇都减少了高压灭菌引起的粘度损失，并对溶液的热凝胶化性质和凝胶的压缩性能产生了积极影响。在葡萄糖存在下进行蒸汽灭菌导致溶液粘度和凝胶强度大幅增加。这与强烈的变色有关，表明体系发生了化学变化。PEG是防止壳聚糖链水解降解最有效的试剂。无论是否存在添加剂，γ射线辐照都会大幅降低壳聚糖溶液的粘度，即使在-80℃下进行灭菌也是如此。此外，热凝胶化性质发生了显著改变，因此，γ射线辐照不是壳聚糖溶液灭菌的合适方法。总之，多元醇可能是一种有用的添加剂，可使蒸汽灭菌后壳聚糖-GP的粘弹性和机械性能最大化。
