牛乳过敏患者中牛乳α(s2)-酪蛋白上连续IgE结合表位的鉴定

Identification of sequential IgE-binding epitopes on bovine alpha(s2)-casein in cow's milk allergic patients.

作者信息

Busse Paula J, Järvinen Kirsi-Marjut, Vila Leticia, Beyer Kirsten, Sampson Hugh A

机构信息

Department of Pediatrics, Division of Allergy and Immunology, Jaffe Institute for Food Allergy, The Mount Sinai School of Medicine, 1 Gustave L. Levy Place, New York, NY 10029-6574, USA.

出版信息

Int Arch Allergy Immunol. 2002 Sep;129(1):93-6. doi: 10.1159/000065178.

DOI:10.1159/000065178

PMID:12373003

Abstract

BACKGROUND

Caseins are the major allergens responsible for cow's milk allergy (CMA). We have previously identified the IgE-binding epitopes of the major cow's milk (CM) proteins except for alpha(s2)-casein.

METHODS

Overlapping decapeptides representing the entire length of alpha(s2)-casein were synthesized on a cellulose-derivatized membrane. Sera from 13 CM-allergic children, 4-15 years of age, with a median level of CM-specific IgE >100 kU/l (range 33.7 to > 100 kU/l) were used to identify IgE-binding epitopes.

RESULTS

Four major and six minor sequential IgE-binding regions were identified on alpha(s2)-casein. The first major region is located in the middle of the protein at amino acids (AA) 83-100, and the other three major regions are located in the carboxy terminal portion of the protein at AA 143-158, 157-172 and 165-188. The minor IgE-binding regions were identified at AA 31-44, 43-56, 93-106, 105-114, 117-128, and 191-200.

CONCLUSION

We identified 10 sequential IgE-binding regions on alpha(s2)-casein and performed the first crucial step in the development of immunotherapeutic interventions for CMA.

摘要

背景

酪蛋白是导致牛奶过敏（CMA）的主要过敏原。我们之前已鉴定出除α(s2)-酪蛋白外的主要牛奶（CM）蛋白的IgE结合表位。

方法

在纤维素衍生膜上合成代表α(s2)-酪蛋白全长的重叠十肽。使用来自13名4至15岁的CM过敏儿童的血清，其CM特异性IgE中位数水平>100 kU/l（范围33.7至>100 kU/l）来鉴定IgE结合表位。

结果

在α(s2)-酪蛋白上鉴定出四个主要和六个次要的连续IgE结合区域。第一个主要区域位于蛋白质中部的氨基酸（AA）83 - 100处，其他三个主要区域位于蛋白质的羧基末端部分，分别在AA 143 - 用158、157 - 172和165 - 188处。次要的IgE结合区域在AA 31 - 44、43 - 56、93 - 106、105 - 114、117 - 128和191 - 200处被鉴定。