Satta L, Antonelli F, Belli M, Sapora O, Simone G, Sorrentino E, Tabocchini M A, Amicarelli F, Ara C, Cerù M P, Colafarina S, Conti Devirgiliis L, De Marco A, Balata M, Falgiani A, Nisi S
Energetics Department, Rome University "La Sapienza", Via A. Scarpa 14, 00161 Rome Italy,
Radiat Environ Biophys. 2002 Sep;41(3):217-24. doi: 10.1007/s00411-002-0159-2. Epub 2002 Aug 8.
We present the results of an experiment aimed at comparing the effects of different background radiation environments on metabolism and responses to gamma-rays and cycloheximide of cultured mammalian cells. Chinese hamster V79 cells were maintained in exponential growth in parallel for up to 9 months at the Istituto Superiore di Sanità (ISS) and at the INFN-Gran Sasso underground Laboratory (LNGS) where exposure due to gamma-rays and to radon was reduced by factors of about 70 and 25, respectively. After 9 months the cells grown at the LNGS (cumulative gamma dose about 30 microGy, average radon concentration around 5 Bq/m(3)), compared to the cells grown at the ISS (cumulative gamma-ray dose about 2 mGy, average radon concentration around 120 Bq/m(3)), exhibited i). a significant increase of the cell density at confluence, ii). a significantly higher capacity to scavenge organic and inorganic hydroperoxides but a reduced scavenging capacity towards superoxide anions and iii). an increase in both the basal hprt mutation frequency and sensitivity to the mutagenic effect of gamma-rays. The cells grown at the LNGS also showed a greater apoptotic sensitivity starting at the third month of culture, that was no longer detected after 9 months. Overall, these data suggest a role of background ionizing radiation in determining an adaptive response, although they cannot be considered conclusive.
我们展示了一项实验的结果，该实验旨在比较不同背景辐射环境对培养的哺乳动物细胞代谢以及对伽马射线和环己酰亚胺反应的影响。中国仓鼠V79细胞在意大利国家高等卫生研究院（ISS）和意大利国家核物理研究所大萨索山地下实验室（LNGS）中平行维持指数生长长达9个月，在LNGS中，伽马射线和氡的暴露分别降低了约70倍和25倍。9个月后，与在ISS生长的细胞（累积伽马射线剂量约2毫戈瑞，平均氡浓度约120贝克勒尔/立方米）相比，在LNGS生长的细胞（累积伽马剂量约30微戈瑞，平均氡浓度约5贝克勒尔/立方米）表现出：i）汇合时细胞密度显著增加；ii）清除有机和无机氢过氧化物的能力显著更高，但对超氧阴离子的清除能力降低；iii）基础次黄嘌呤-鸟嘌呤磷酸核糖转移酶（hprt）突变频率增加以及对伽马射线诱变作用的敏感性增加。在LNGS生长的细胞从培养第三个月开始还表现出更高的凋亡敏感性，9个月后不再检测到这种敏感性。总体而言，这些数据表明背景电离辐射在确定适应性反应中起作用，尽管这些数据不能被视为结论性的。
