Rahman Nafisur, Hoda Md Nasrul
Analytical Research Division, Department of Chemistry, Aligarh Muslim University, Aligarh-202002, India.
Anal Bioanal Chem. 2002 Oct;374(3):484-9. doi: 10.1007/s00216-002-1521-6. Epub 2002 Sep 7.
A new spectrophotometric method is described for the determination of verapamil hydrochloride, based on its oxidation with chloramine-T in hydrochloric acid medium. It produces a yellow colored compound with maximum absorbance at 425 nm. Beer's law was obeyed in the concentration range 0-340 micro g mL(-1) with molar absorptivity 2 x 10(3) L mol(-1) cm(-1) and RSD 0.3-0.82%. All variables were studied to optimize the reaction conditions. No interferences were observed from the common excipients present in the formulations. The method has been applied successfully to the determination of the drug in pharmaceutical preparations. Statistical comparison of the results with those from the reference method reveals excellent agreement and confirms that accuracy and precision are not significantly different.
描述了一种用于测定盐酸维拉帕米的新分光光度法，该方法基于在盐酸介质中用氯胺 - T对其进行氧化。它产生一种黄色化合物，在425 nm处有最大吸收。在0 - 340μg mL(-1)的浓度范围内符合比尔定律，摩尔吸光系数为2×10(3) L mol(-1) cm(-1)，相对标准偏差为0.3 - 0.82%。研究了所有变量以优化反应条件。未观察到制剂中常见辅料的干扰。该方法已成功应用于药物制剂中药物的测定。结果与参考方法的统计比较显示出极好的一致性，并证实准确性和精密度没有显著差异。
