Walash M I, Belal F, El-Enany N, Abdelal A A
Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura, Egypt.
Int J Biomed Sci. 2009 Jun;5(2):146-57.
A simple and sensitive kinetic spectrofluorometric method was developed for the determination of some calcium channel blockers namely, verapamil hydrochloride, diltiazem hydrochloride, nicardipine hydrochloride and flunarizine. The method is based upon oxidation of the studied drugs with cerium (IV) ammonium sulphate in acidic medium. The fluorescence of the produced Ce (III) was measured at 365 nm after excitation at 255 nm. The different experimental parameters affecting the development and stability of the reaction product were carefully studied and optimized. The fluorescence-concentration plots were rectilinear for all the studied compounds over the concentration range of 0.01 to 0.12 μg mL(-1). The limits of detections for the studied compounds ranged from 2.93 × 10(-3) to 0.012 μg mL(-1) and limits of quantification from 9.76 × 10(-3) to 0.04 μg mL(-1) were obtained. The method was successfully applied to the analysis of commercial tablets. The results obtained were in good agreement with those obtained with reference methods.
建立了一种简单灵敏的动力学荧光分光光度法，用于测定某些钙通道阻滞剂，即盐酸维拉帕米、盐酸地尔硫䓬、盐酸尼卡地平及氟桂利嗪。该方法基于在酸性介质中，用硫酸铈（IV）铵氧化所研究的药物。在255nm激发后，于365nm处测量生成的Ce（III）的荧光。仔细研究并优化了影响反应产物形成和稳定性的不同实验参数。在所研究的所有化合物浓度范围为0.01至0.12μg mL(-1)时，荧光-浓度曲线呈线性。所研究化合物的检测限范围为2.93×10(-3)至0.012μg mL(-1)，定量限范围为9.76×10(-3)至0.04μg mL(-1)。该方法成功应用于市售片剂的分析。所得结果与参考方法获得的结果高度一致。
