Kaneider Nicole C, Rabensteiner Andrea, Reinisch Christina M, Dunzendorfer Stefan, Wiedermann Christian J
Division of General Internal Medicine, Department of Internal Medicine, University of Innsbruck, Anichstrasse 35, 6020 Innsbruck, Austria.
Intensive Care Med. 2002 Oct;28(10):1447-52. doi: 10.1007/s00134-002-1432-1. Epub 2002 Aug 1.
Antithrombin exerts direct effects on neutrophils by inhibiting chemokine-induced migration. This study examined the potency of different pharmaceutical antithrombin preparations in inhibiting neutrophil chemotaxis toward interleukin 8.
Cell migration was tested by the leading front assay in modified Boyden microchemotaxis chambers bearing nitrocellulose filters. Human neutrophils were incubated with six different antithrombin concentrates or an immunopurified antithrombin preparation at concentrations of 1 micro IUeth-5 IU/ml.
All antithrombin concentrates irrespective of the pharmaceutical source deactivated neutrophil chemotaxis. At concentrations below 100 mIU/ml neutrophil chemotaxis toward interleukin 8 was decreased by the antithrombin preparations with varying potency, but at 1 mIU/ml no significant differences were observed.
As the ability of antithrombin to deactivate neutrophil chemotaxis toward interleukin 8 shows differences depending on the source of commercial antithrombin, these results suggest that at equivalent WHO standard concentrations clinical antithrombin concentrates may differ in anti-inflammatory potential.
抗凝血酶通过抑制趋化因子诱导的迁移对中性粒细胞产生直接作用。本研究检测了不同药物抗凝血酶制剂抑制中性粒细胞向白细胞介素8趋化的效力。
在装有硝酸纤维素滤膜的改良博伊登微量趋化小室中，通过前沿检测法检测细胞迁移。将人类中性粒细胞与六种不同的抗凝血酶浓缩物或一种免疫纯化的抗凝血酶制剂在浓度为1微国际单位/毫升至5国际单位/毫升的条件下孵育。
所有抗凝血酶浓缩物，无论其药物来源如何，均能使中性粒细胞趋化失活。在浓度低于100毫国际单位/毫升时，抗凝血酶制剂以不同效力降低了中性粒细胞向白细胞介素8的趋化，但在1毫国际单位/毫升时未观察到显著差异。
由于抗凝血酶使中性粒细胞向白细胞介素8趋化失活的能力因商业抗凝血酶的来源不同而存在差异，这些结果表明，在世界卫生组织标准等效浓度下，临床抗凝血酶浓缩物的抗炎潜力可能有所不同。
