Rot A
Sandoz Forschungsinstitut, Vienna, Austria.
Eur J Immunol. 1993 Jan;23(1):303-6. doi: 10.1002/eji.1830230150.
The role of leukocyte migration induced by the substrate-bound attractants is obscured by the inability of standard methods for the study of leukocyte migration to dissociate chemotaxis and haptotaxis, migration in response to soluble and surface-bound gradients of attractants. Here we show that the gradient of neutrophil attractant/activation protein-1 (interleukin-8, NAP-1/IL-8) induces directed in vitro neutrophil migration when bound to the polycarbonate filter. In addition, we suggest that haptotaxis is responsible for neutrophil migration induced by NAP-1/IL-8 in standard Boyden-type chemotaxis assays and, in light of the ability of NAP-1/IL-8 to bind to the endothelial cell surface and the extracellular matrix, could also be of great significance in vivo.
由于研究白细胞迁移的标准方法无法区分趋化作用和触觉作用（即对可溶性和表面结合的趋化因子梯度的迁移反应），因此底物结合的趋化因子诱导的白细胞迁移作用变得模糊不清。在此我们表明，中性粒细胞趋化因子/激活蛋白-1（白细胞介素-8，NAP-1/IL-8）的梯度与聚碳酸酯滤膜结合时，可在体外诱导中性粒细胞定向迁移。此外，我们认为在标准的博伊登型趋化分析中，触觉作用是由NAP-1/IL-8诱导的中性粒细胞迁移的原因，鉴于NAP-1/IL-8能够结合内皮细胞表面和细胞外基质，其在体内也可能具有重要意义。
