Ikeguchi M, Kaibara N
First Department of Surgery, Faculty of Medicine, Tottori University, 36-1 Nishi-cho, Yonago 683-8504, Japan.
Br J Cancer. 2002 Oct 7;87(8):883-7. doi: 10.1038/sj.bjc.6600546.
Oesophageal squamous cell carcinoma is one of the most malignant tumours. To identify patients with a high risk of recurrence of oesophageal squamous cell carcinoma, we investigated the prognostic significance of survivin mRNA expression in oesophageal squamous cell carcinoma, which has recently been reported to be a good marker for unfavourable prognosis in various tumours. Tumours and non-cancerous epitheliums adjacent to tumours were obtained by surgical resection from 57 patients with oesophageal squamous cell carcinoma. Expression levels of survivin and glyceraldehyde-3-phosphate dehydrogenase mRNA were analysed quantitatively by real-time reverse transcriptase polymerase chain reaction (RT-PCR). The survivin/glyceraldehyde-3-phosphate dehydrogenase ratios of tumours were higher than those of non-cancerous tissues (P=0.0003). Tumour-survivin/glyceraldehyde-3-phosphate dehydrogenase ratio did not correlate with histologic type, lymph node metastasis, and stage of tumours. In 53 surviving patients, the 5-year survival rate of 17 patients with high survivin mRNA expressed oesophageal squamous cell carcinoma (14.1%) was significantly poorer than that of 36 with low survivin mRNA expressed oesophageal squamous cell carcinoma (46.8%, P=0.0018). In these patients, tumour-survivin mRNA expression was recognised as a good marker of cancer recurrence independently from tumour stage. These findings indicate that survivin mRNA expression in oesophageal squamous cell carcinoma may be a good biomarker for identifying patients with high risk of cancer recurrence.
食管鳞状细胞癌是最恶性的肿瘤之一。为了识别食管鳞状细胞癌复发风险高的患者，我们研究了生存素mRNA表达在食管鳞状细胞癌中的预后意义，最近有报道称其是各种肿瘤预后不良的良好标志物。通过手术切除从57例食管鳞状细胞癌患者中获取肿瘤组织及肿瘤旁非癌上皮组织。采用实时逆转录聚合酶链反应（RT-PCR）定量分析生存素和甘油醛-3-磷酸脱氢酶mRNA的表达水平。肿瘤组织的生存素/甘油醛-3-磷酸脱氢酶比值高于非癌组织（P=0.0003）。肿瘤生存素/甘油醛-3-磷酸脱氢酶比值与肿瘤的组织学类型、淋巴结转移及分期无关。在53例存活患者中，17例生存素mRNA高表达的食管鳞状细胞癌患者的5年生存率（14.1%）显著低于36例生存素mRNA低表达的食管鳞状细胞癌患者（46.8%，P=0.0018）。在这些患者中，肿瘤生存素mRNA表达被认为是独立于肿瘤分期的癌症复发的良好标志物。这些发现表明，食管鳞状细胞癌中生存素mRNA表达可能是识别癌症复发高风险患者的良好生物标志物。
