Liggins J, Mulligan A, Runswick S, Bingham S A
Medical Research Council Dunn Human Nutrition Unit, Hills Road, Cambridge, UK.
Eur J Clin Nutr. 2002 Oct;56(10):961-6. doi: 10.1038/sj.ejcn.1601419.
To analyse 75 cereals and three soy flours commonly eaten in Europe for the phytoestrogens daidzein and genistein.
The phytoestrogens daidzein and genistein were extracted from dried foods, and the two isoflavones quantified after hydrolytic removal of any conjugated carbohydrate. Completeness of extraction and any procedural losses of the isoflavones were accounted for using synthetic daidzin (7-O-glucosyl-4'-hydroxyisoflavone) and genistin (7-O-glucosyl-4'5-dihydroxyisoflavone) as internal standards.
Foods from the Cambridge UK area were purchased, prepared for eating, which included cooking if necessary, and freeze dried. Three stock soy flours were also analysed.
Eighteen of the foods assayed contained trace or no detectable daidzein or genistein. The soy flours were rich sources, containing 1639-2117 mg/kg. The concentration of the two isoflavones in the remaining foods ranged from 33 to 11,873 micro g/kg.
These analyses will supply useful information to investigators determining the intake of phytoestrogens in cereal products in order to relate intakes to potential biological activities.
This work was supported by the United Kingdom Medical Research Council, Ministry of Agriculture Fisheries and Food (contract FS2034) and the United States of America Army (contract DAMD 17-97-1-7028).
分析欧洲常见的75种谷物和3种大豆粉中的植物雌激素黄豆苷元和染料木黄酮。
从干燥食品中提取植物雌激素黄豆苷元和染料木黄酮，在水解去除任何共轭碳水化合物后对这两种异黄酮进行定量。使用合成黄豆苷（7 - O - 葡萄糖基 - 4'-羟基异黄酮）和染料木苷（7 - O - 葡萄糖基 - 4'5 - 二羟基异黄酮）作为内标，计算提取的完整性和异黄酮的任何操作损失。
购买英国剑桥地区的食品，进行食用准备，必要时包括烹饪，然后冷冻干燥。还对三种大豆粉原料进行了分析。
所检测的食品中有18种含有微量或未检测到黄豆苷元或染料木黄酮。大豆粉是丰富的来源，含量为1639 - 2117毫克/千克。其余食品中这两种异黄酮的浓度范围为33至11873微克/千克。
这些分析将为研究人员提供有用信息，以确定谷物产品中植物雌激素的摄入量，从而将摄入量与潜在的生物活性联系起来。
这项工作得到了英国医学研究理事会、农业渔业和食品部（合同编号FS2034）以及美国陆军（合同编号DAMD 17 - 97 - 1 - 7028）的支持。
