Compton Mark M, Lapp Stacey A, Pedemonte Ron
Department of Poultry Science, The University of Georgia, Athens, GA 30602-2772, USA.
Electrophoresis. 2002 Sep;23(19):3262-5. doi: 10.1002/1522-2683(200210)23:19<3262::AID-ELPS3262>3.0.CO;2-8.
Molecular weight marker proteins are routinely used in sodium dodecyl sulfate-polyacrylamide gel electrophoresis to estimate the relative molecular mass of specific proteins within a sample. This report describes a simple procedure for the generation of multicolored molecular weight proteins using a variety of Remazol-reactive textile dyes. These multicolored proteins provide a set of unambiguous markers for gel electrophoresis. Furthermore, the colored markers can be used in conjunction with Western blotting techniques to provide a visual display of marker proteins on the transfer membrane.
