Liu Shuhui, Li Qianfeng, Chen Xingguo, Hu Zhide
Department of Chemistry, Lanzhou University, Lanzhou, P. R. C.
Electrophoresis. 2002 Sep;23(19):3392-7. doi: 10.1002/1522-2683(200210)23:19<3392::AID-ELPS3392>3.0.CO;2-X.
A simple and rapid capillary zone electrophoresis method was developed for the separation of the main alkaloids from Sophora flavescens Ait. with the optimum buffer solution containing 110 mM NaH(2)PO(4) and 15% 2-propanol (pH 3.0). The field-amplified sample stacking (FASS) technique was applied to the on-line concentration of the alkaloids. The data presented in this work demonstrate that the use of a short water plug at the column inlet is essential for improving the reproducibility of FASS with electro-injection, and that the water plug injection time affected the sensitivity significantly. The sample concentration was further increased by about 2-3-fold by the introduction of a relatively longer water plug. With this stacking measure, the concentration sensitivity was about 3-4 orders of magnitude higher than in hydrodynamic injection.
建立了一种简单快速的毛细管区带电泳方法，用于分离苦参中的主要生物碱。最佳缓冲溶液含有110 mM磷酸二氢钠和15%异丙醇（pH 3.0）。采用场放大进样（FASS）技术对生物碱进行在线富集。本文给出的数据表明，在柱入口处使用短水塞对于提高电进样FASS的重现性至关重要，并且水塞进样时间对灵敏度有显著影响。通过引入相对较长的水塞，样品浓度进一步提高了约2 - 3倍。采用这种富集方法，浓度灵敏度比流体动力进样高约3 - 4个数量级。
