College of Science, Northwest A&F University, No. 3 Taicheng Road, Yangling, Shaanxi 712100, China.
J Pharm Biomed Anal. 2012 Jan 25;58:146-51. doi: 10.1016/j.jpba.2011.09.014. Epub 2011 Sep 22.
The extraction and determination of cytisine, sophocarpine, matrine, sophoridine and oxymatrine in Sophora flavescens Ait. were performed using subcritical water extraction and capillary electrophoresis with field-amplified sample stacking. The effects of extraction temperature, pressure, time and cycle number on the extraction yields were investigated systematically for accelerated solvent extraction with ethanol (ASE) and accelerated solvent extraction with water (subcritical water extraction, SWE). The extraction yields obtained using SWE, ASE, water ultrasonic extraction and chloroform soaking extraction methods were compared. The electrophoresis separation buffer was monosodium phosphate (pH 3.0; 110 mM)-isopropanol (85:15, v/v). The effect of phosphoric acid added to the sample matrix on the reproducibility of the peak heights of the analytes was also examined. Cytisine, sophoridine and oxymatrine showed good linearity (R(2)>0.999) within 0.125-4.0 μg/mL, and sophocarpine and matrine exhibited good linearity (R(2)>0.998) within 0.0625-2.0 μg/mL, with the detection limits in the range of 0.004-0.0013 μg/mL. The five alkaloid contents in medicinal plants from different sources and Sophora instant granule were determined and compared.
采用亚临界水提取和场放大样品堆积毛细管电泳法，从苦参中提取并测定了野靛碱、槐果碱、苦参碱、槐定碱和氧化苦参碱。系统研究了乙醇加速溶剂提取（ASE）和水加速溶剂提取（亚临界水提取，SWE）中提取温度、压力、时间和循环数对提取产率的影响。比较了 SWE、ASE、水超声提取和氯仿浸泡提取方法的提取产率。电泳分离缓冲液为单磷酸钠（pH 3.0；110 mM）-异丙醇（85:15，v/v）。还考察了向样品基质中添加磷酸对分析物峰高重现性的影响。野靛碱、槐定碱和氧化苦参碱在 0.125-4.0 μg/mL 范围内具有良好的线性（R(2)>0.999），槐果碱和苦参碱在 0.0625-2.0 μg/mL 范围内具有良好的线性（R(2)>0.998），检测限在 0.004-0.0013 μg/mL 范围内。测定并比较了不同来源药用植物和苦参速溶颗粒中的五种生物碱含量。
