Kurosou Kazuko, Murakami Shigeru, Jinnai Mutsuo, Koba Hiroyuki, Imai Yuuko, Yamada Takashi, Ueda Yoshihiko, Mori Yoshiomi
Department of Pathology, Dokkyo University School of Medicine, Koshigaya Hospital, Koshigaya 343-8555.
Rinsho Byori. 2002 Aug;50(8):830-4.
We investigated fluorescence in situ hybridization (FISH) analysis using microwave irradiation in formalin-fixed, paraffin-embedded tissue sections of breast fibroadenoma. Higher percentage of cells with 2 signal copies of chromosome 3 centromere could be obtained in the condition of 5 microns thick sections, when we counted cells of more than 4 microns of nuclei in thickness. This method showed about the same results as FISH using cells separated from the same tissues. Percentage of cells with 2 signal copies of chromosome 17 centromere in 14 cases was 80.6 +/- 4.0% (Mean +/- S.D.). This method is expected in the application of the prognosis estimation of the breast cancer.
我们研究了在福尔马林固定、石蜡包埋的乳腺纤维腺瘤组织切片中使用微波辐射进行荧光原位杂交（FISH）分析。当我们对核厚度超过4微米的细胞进行计数时，在5微米厚切片的条件下可获得更高比例的具有3号染色体着丝粒2个信号拷贝的细胞。该方法显示的结果与使用从相同组织中分离的细胞进行FISH的结果大致相同。14例中具有17号染色体着丝粒2个信号拷贝的细胞百分比为80.6 +/- 4.0%（平均值 +/- 标准差）。该方法有望应用于乳腺癌的预后评估。
