Uhara Miho, Matsuda Kazuyuki, Shimojyo Yasuyo, Hidaka Eiko, Kumagai Toshiko, Uehara Takeshi, Sano Kenji, Honda Takayuki
Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto 390-8621, Japan.
Rinsho Byori. 2010 Jan;58(1):25-9.
The gene amplification of human epidermal growth factor receptor (HER-2) detected by fluorescence in situ hybridization (FISH) help to select breast cancer patients who could benefit from therapeutic strategies targeting HER-2, trastuzumab. For formalin-fixed paraffin-embedded tissue specimens, the preprocessing procedure, which is consisted of heat-treatment and protease digestion, is necessary to detect probe specific signal by FISH. We compared the findings of two commercial kits for HER-2 (Histra HER2 FISH Kit [JOKOH CO.LTD] and Pathvysion HER-2 DNA Probe Kit [Abbot Molecular CO. LTD]) using 9 breast cancer specimens, which were fixed in formalin for various durations after the surgical extirpation or biopsy. The two kits showed the same results that HER-2/17 centromere (17cen) signal ratio was within normal rage (< 2.0) in 7 cases among 9 and was over 2.0 in remaining 2. Histra could obtain sufficient signals on both surgical and needle biopsy speciments after the same pretreatments. PathVysion, on the other hand, require a different protease or modification of protease digestion time. Furthermore, the specific signals for HER-2 and 17cen could be observed more clearly by Histra than PathVysion under fluorescensce microscope. Histra is more suitable for routine examination because of its simple and constant-processing procedure.
通过荧光原位杂交（FISH）检测人表皮生长因子受体（HER-2）的基因扩增，有助于筛选出可从针对HER-2的治疗策略（曲妥珠单抗）中获益的乳腺癌患者。对于福尔马林固定石蜡包埋的组织标本，由热处理和蛋白酶消化组成的预处理程序对于通过FISH检测探针特异性信号是必要的。我们使用9例乳腺癌标本比较了两种用于HER-2的商业试剂盒（Histra HER2 FISH试剂盒[JOKOH有限公司]和Pathvysion HER-2 DNA探针试剂盒[雅培分子有限公司]）的检测结果，这些标本在手术切除或活检后用福尔马林固定了不同时长。两种试剂盒显示了相同的结果，9例中有7例HER-2/17号染色体着丝粒（17cen）信号比值在正常范围内（<2.0），其余2例超过2.0。经过相同的预处理后，Histra在手术标本和针吸活检标本上都能获得足够的信号。另一方面，PathVysion需要不同的蛋白酶或调整蛋白酶消化时间。此外，在荧光显微镜下，Histra比PathVysion能更清晰地观察到HER-2和17cen的特异性信号。由于其处理程序简单且固定，Histra更适合常规检查。
