[辅助生殖项目中人类卵母细胞和胚胎透明带的分析]
[Analysis of the zona pellucida in human oocytes and embryos in an assisted reproduction program].
作者信息
Krizanovská K, Ulcová-Gallová Z, Bouse V, Svábek L, Rokyta P, Suchá R, Rokyta Z
机构信息
Gynekologicko-porodnická klinika UK, LF a FN v Plzni.
出版信息
Ceska Gynekol. 2002 Jul;67(4):197-202.
OBJECTIVE
Our aim was to design simple method for quantitative evaluation of oocyte and embryo morphology in in vitro fertilization (IVF) programme efforting of embryo implantation prediction to be able to set the optimal number of embryos for transfer for given specific case. This study deals with zona pellucida (ZP)--the one interesting and for oocyte unique structure. The work is the part of comprehensive research of oocyte, zygote and embryo morphology.
DESIGN
Prospective study.
SETTING
Center of Assisted Reproduction, Department of Gynaecology and Obstetrics, Medical Faculty of Charles University, Pilsen.
METHODS
The study includes analysis of 66 microphotography pictures of oocytes, zygotes and embryos of patients who were treated by IVF on our department between September and December 2000. The microphotographs were taken by digital microphotographic system. Analysis of zona pellucida parameters was conducted by image analysis software called AnalySIS, Soft Imaging System GmbH.
RESULTS
It was found out the average width of the zona pellucida 18.4 microns +/- 2.1 microns, the minimum width was 12.3 microns a maximum 23.1 microns.
CONCLUSION
There was found significant narrower zona pellucida in women who conceived in this IVF cycle then in the women who did not.
目的
我们的目标是设计一种简单方法，用于在体外受精（IVF）程序中对卵母细胞和胚胎形态进行定量评估，以努力预测胚胎着床情况，从而能够为特定病例确定最佳的移植胚胎数量。本研究涉及透明带（ZP）——一种有趣且对于卵母细胞而言独特的结构。这项工作是对卵母细胞、受精卵和胚胎形态进行全面研究的一部分。
设计
前瞻性研究。
地点
查尔斯大学医学院妇产科辅助生殖中心，皮尔森。
方法
该研究包括对2000年9月至12月在我们科室接受IVF治疗的患者的66张卵母细胞、受精卵和胚胎的显微照片进行分析。显微照片由数字显微摄影系统拍摄。使用名为AnalySIS的图像分析软件（Soft Imaging System GmbH）对透明带参数进行分析。
结果
发现透明带的平均宽度为18.4微米±2.1微米，最小宽度为12.3微米，最大宽度为23.1微米。
结论
发现在此IVF周期中受孕的女性的透明带明显比未受孕女性的窄。
Zotero 插件