Diaz Emilce S, Rettori Valeria, Suescun Maria O, Lustig Livia, McCann Samuel M, Denduchis Berta
Centro de Investigaciones en Reproducción, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
Endocrine. 2002 Jul;18(2):185-9. doi: 10.1385/ENDO:18:2:185.
We previously demonstrated that laminin, a component of basement membranes, modulates pituitary hormone secretion. In the present study, we evaluated the effect of type IV collagen, another component of this membrane, on the release of prolactin (PRL) by anterior pituitary gland from adult male rats. Hemipituitaries were incubated for 3 h with type IV collagen or antibodies against it and PRL release was studied. Rabbit IgG to type IV collagen at concentrations of 10(-7) - 10(-5) M had a significant stimulatory effect on PRL release, in comparison to normal rabbit serum IgG or medium alone used as controls. Type IV collagen induced a significant inhibitory effect on basal release of PRL at a concentration of 30 microg/mL. A slight decrease in PRL release was detected in thyrotropin-releasing hormone-stimulated hemipituitaries incubated with type IV collagen at all concentrations used. These results suggest that type IV collagen, similar to laminin-1, modulates PRL released from hemipituitaries, in vitro.
我们之前证明，作为基底膜成分之一的层粘连蛋白可调节垂体激素分泌。在本研究中，我们评估了该膜的另一种成分IV型胶原对成年雄性大鼠垂体前叶催乳素（PRL）释放的影响。将半垂体与IV型胶原或其抗体孵育3小时，并研究PRL释放情况。与用作对照的正常兔血清IgG或单独的培养基相比，浓度为10(-7)-10(-5)M的抗IV型胶原兔IgG对PRL释放具有显著的刺激作用。IV型胶原在浓度为30μg/mL时对PRL的基础释放具有显著的抑制作用。在所有使用的浓度下，与IV型胶原孵育的促甲状腺激素释放激素刺激的半垂体中，PRL释放均有轻微下降。这些结果表明，IV型胶原与层粘连蛋白-1类似，在体外可调节半垂体释放PRL。
