• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

大肠杆菌杂交簇蛋白的羟胺还原酶活性

Hydroxylamine reductase activity of the hybrid cluster protein from Escherichia coli.

作者信息

Wolfe Marcus T, Heo Jongyun, Garavelli John S, Ludden Paul W

机构信息

Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA.

出版信息

J Bacteriol. 2002 Nov;184(21):5898-902. doi: 10.1128/JB.184.21.5898-5902.2002.

DOI:10.1128/JB.184.21.5898-5902.2002
PMID:12374823
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC135376/
Abstract

The hybrid cluster protein (HCP; formerly termed the prismane protein) has been extensively studied due to its unique spectroscopic properties. Although the structural and spectroscopic characteristics are well defined, its enzymatic function, up to this point, has remained unidentified. While it was proposed that HCP acts in some step of nitrogen metabolism, a specific role for this enzyme remained unknown. Recent studies of HCP purified from Escherichia coli have identified a novel hydroxylamine reductase activity. These data reveal the ability of HCP to reduce hydroxylamine in vitro to form NH(3) and H(2)O. Further biochemical analyses were completed in order to determine the effects of various electron donors, different pH levels, and the presence of CN(-) on in vitro hydroxylamine reduction.

摘要

杂合簇蛋白（HCP；以前称为棱柱烷蛋白）因其独特的光谱性质而受到广泛研究。尽管其结构和光谱特征已得到明确界定，但到目前为止，其酶功能仍未确定。虽然有人提出HCP在氮代谢的某些步骤中起作用，但该酶的具体作用仍然未知。最近对从大肠杆菌中纯化的HCP的研究确定了一种新的羟胺还原酶活性。这些数据揭示了HCP在体外将羟胺还原形成NH(3)和H(2)O的能力。为了确定各种电子供体、不同pH水平以及CN(-)的存在对体外羟胺还原的影响，进一步完成了生化分析。

相似文献

1
Hydroxylamine reductase activity of the hybrid cluster protein from Escherichia coli.大肠杆菌杂交簇蛋白的羟胺还原酶活性
J Bacteriol. 2002 Nov;184(21):5898-902. doi: 10.1128/JB.184.21.5898-5902.2002.
2
The roles of the hybrid cluster protein, Hcp and its reductase, Hcr, in high affinity nitric oxide reduction that protects anaerobic cultures of Escherichia coli against nitrosative stress.杂合簇蛋白Hcp及其还原酶Hcr在高亲和力一氧化氮还原中的作用，这种作用可保护大肠杆菌的厌氧培养物免受亚硝化应激。
Mol Microbiol. 2016 Jun;100(5):877-92. doi: 10.1111/mmi.13356. Epub 2016 Mar 22.
3
Mössbauer studies of Escherichia coli sulfite reductase complexes with carbon monoxide and cyanide. Exchange coupling and intrinsic properties of the [4Fe-4S] cluster.大肠杆菌亚硫酸盐还原酶与一氧化碳和氰化物复合物的穆斯堡尔研究。[4Fe-4S]簇的交换耦合和内在性质。
J Biol Chem. 1983 Sep 25;258(18):11157-64.
4
EPR spectroscopy of putative enzyme intermediates in the NO reductase and the auto-nitrosylation reaction of Desulfovibrio vulgaris hybrid cluster protein.EPR 光谱学在假定的酶中间体中的应用，这些中间体存在于一氧化氮还原酶和脱硫弧菌杂种[FeFe]-氢化酶中自硝化反应。
FEBS Lett. 2019 Nov;593(21):3075-3083. doi: 10.1002/1873-3468.13539. Epub 2019 Jul 24.
5
Converting the NiFeS carbon monoxide dehydrogenase to a hydrogenase and a hydroxylamine reductase.将镍铁硫一氧化碳脱氢酶转化为氢化酶和羟胺还原酶。
J Bacteriol. 2002 Nov;184(21):5894-7. doi: 10.1128/JB.184.21.5894-5897.2002.
6
Hydroxylamine assimilation by Rhodobacter capsulatus E1F1. requirement of the hcp gene (hybrid cluster protein) located in the nitrate assimilation nas gene region for hydroxylamine reduction.荚膜红细菌E1F1对羟胺的同化作用。位于硝酸盐同化nas基因区域的hcp基因（杂合簇蛋白）对羟胺还原的需求。
J Biol Chem. 2004 Oct 29;279(44):45485-94. doi: 10.1074/jbc.M404417200. Epub 2004 Aug 18.
7
The hybrid-cluster protein ('prismane protein') from Escherichia coli. Characterization of the hybrid-cluster protein, redox properties of the [2Fe-2S] and [4Fe-2S-2O] clusters and identification of an associated NADH oxidoreductase containing FAD and [2Fe-2S].来自大肠杆菌的杂合簇蛋白（“棱柱烷蛋白”）。杂合簇蛋白的特性、[2Fe-2S]和[4Fe-2S-2O]簇的氧化还原性质以及一种含有FAD和[2Fe-2S]的相关NADH氧化还原酶的鉴定。
Eur J Biochem. 2000 Feb;267(3):666-76. doi: 10.1046/j.1432-1327.2000.01032.x.
8
Transcriptional regulation of a hybrid cluster (prismane) protein.一种杂合簇（棱柱烷）蛋白的转录调控
Biochem Soc Trans. 2005 Feb;33(Pt 1):195-7. doi: 10.1042/BST0330195.
9
Release of nitric oxide by the Escherichia coli YtfE (RIC) protein and its reduction by the hybrid cluster protein in an integrated pathway to minimize cytoplasmic nitrosative stress.大肠杆菌 YtfE（RIC）蛋白释放的一氧化氮及其在综合途径中被混合簇蛋白还原，以最小化细胞质硝化应激。
Microbiology (Reading). 2018 Apr;164(4):563-575. doi: 10.1099/mic.0.000629. Epub 2018 Mar 1.
10
Mössbauer spectroscopic studies of Escherichia coli sulfite reductase. Evidence for coupling between the siroheme and Fe4S4 cluster prosthetic groups.大肠杆菌亚硫酸盐还原酶的穆斯堡尔光谱研究。西罗血红素与Fe4S4簇辅基之间偶联的证据。
J Biol Chem. 1981 Mar 10;256(5):2098-101.

引用本文的文献

1
Metabolic versatility and nitrate reduction pathways of a new thermophilic bacterium of the Deferrivibrionaceae: Deferrivibrio metallireducens sp. nov isolated from hot sediments of Vulcano Island, Italy.脱铁弧菌科一种新嗜热细菌的代谢多样性和硝酸盐还原途径：从意大利武尔卡诺岛热沉积物中分离出的新种金属还原脱铁弧菌（Deferrivibrio metallireducens sp. nov）
PLoS One. 2025 Mar 11;20(3):e0315093. doi: 10.1371/journal.pone.0315093. eCollection 2025.
2
Nitrogen Journey in Plants: From Uptake to Metabolism, Stress Response, and Microbe Interaction.植物中的氮素之旅：从吸收到代谢、应对胁迫和微生物互作。
Biomolecules. 2023 Sep 25;13(10):1443. doi: 10.3390/biom13101443.
3
Class III hybrid cluster protein homodimeric architecture shows evolutionary relationship with Ni, Fe-carbon monoxide dehydrogenases.III 类杂合簇蛋白同源二聚体结构与 Ni、Fe-一氧化碳脱氢酶具有进化关系。
Nat Commun. 2023 Sep 14;14(1):5609. doi: 10.1038/s41467-023-41289-4.
4
Insight into the metabolic potential and ecological function of a novel Magnetotactic in coral reef habitat.对珊瑚礁栖息地中一种新型趋磁细菌的代谢潜力和生态功能的洞察。
Front Microbiol. 2023 May 17;14:1182330. doi: 10.3389/fmicb.2023.1182330. eCollection 2023.
5
Structural and biochemical elucidation of class I hybrid cluster protein natively extracted from a marine methanogenic archaeon.从海洋产甲烷古菌中天然提取的I类杂交簇蛋白的结构与生化解析
Front Microbiol. 2023 May 11;14:1179204. doi: 10.3389/fmicb.2023.1179204. eCollection 2023.
6
Antibacterial Components and Modes of the Methanol-Phase Extract from Linn.来自[植物名称未给出，推测应为某植物 Linn. 的甲醇相提取物的抗菌成分及作用方式
Plants (Basel). 2023 Feb 16;12(4):890. doi: 10.3390/plants12040890.
7
A Morphing [4Fe-3S-nO]-Cluster within a Carbon Monoxide Dehydrogenase Scaffold.一氧化碳脱氢酶支架内的一个形态变化的[4Fe-3S-nO]-簇。
Angew Chem Int Ed Engl. 2022 Apr 25;61(18):e202117000. doi: 10.1002/anie.202117000. Epub 2022 Mar 4.
8
Molecular understanding of heteronuclear active sites in heme-copper oxidases, nitric oxide reductases, and sulfite reductases through biomimetic modelling.通过仿生模拟研究细胞色素 c 氧化酶、一氧化氮还原酶和亚硫酸盐还原酶中异核活性部位的分子机制。
Chem Soc Rev. 2021 Mar 1;50(4):2486-2539. doi: 10.1039/d0cs01297a.
9
" Galacturonibacter soehngenii" Shows Acetogenic Catabolism of Galacturonic Acid but Lacks a Canonical Carbon Monoxide Dehydrogenase/Acetyl-CoA Synthase Complex.“索氏半乳糖醛酸杆菌”表现出半乳糖醛酸的产乙酸分解代谢，但缺乏典型的一氧化碳脱氢酶/乙酰辅酶A合成酶复合物。
Front Microbiol. 2020 Jan 29;11:63. doi: 10.3389/fmicb.2020.00063. eCollection 2020.
10
A Complex Interplay between Nitric Oxide, Quorum Sensing, and the Unique Secondary Metabolite Tundrenone Constitutes the Hypoxia Response in .一氧化氮、群体感应和独特的次生代谢物通德雷酮之间的复杂相互作用构成了……中的缺氧反应。
mSystems. 2020 Jan 21;5(1):e00770-19. doi: 10.1128/mSystems.00770-19.

本文引用的文献

1
Converting the NiFeS carbon monoxide dehydrogenase to a hydrogenase and a hydroxylamine reductase.将镍铁硫一氧化碳脱氢酶转化为氢化酶和羟胺还原酶。
J Bacteriol. 2002 Nov;184(21):5894-7. doi: 10.1128/JB.184.21.5894-5897.2002.
2
Redox-dependent CO2 reduction activity of CO dehydrogenase from Rhodospirillum rubrum.红螺菌属红螺菌的一氧化碳脱氢酶的氧化还原依赖性二氧化碳还原活性。
Biochemistry. 2001 Jun 26;40(25):7604-11. doi: 10.1021/bi002554k.
3
Hybrid-cluster protein (HCP) from Desulfovibrio vulgaris (Hildenborough) at 1.6 A resolution.来自普通脱硫弧菌（希登伯勒菌株）的混合簇蛋白（HCP），分辨率为1.6埃。
Biochemistry. 2000 Dec 12;39(49):15044-54. doi: 10.1021/bi001483m.
4
Regulation of biological nitrogen fixation.
J Nutr. 2000 May;130(5):1081-4. doi: 10.1093/jn/130.5.1081.
5
The hybrid-cluster protein ('prismane protein') from Escherichia coli. Characterization of the hybrid-cluster protein, redox properties of the [2Fe-2S] and [4Fe-2S-2O] clusters and identification of an associated NADH oxidoreductase containing FAD and [2Fe-2S].来自大肠杆菌的杂合簇蛋白（“棱柱烷蛋白”）。杂合簇蛋白的特性、[2Fe-2S]和[4Fe-2S-2O]簇的氧化还原性质以及一种含有FAD和[2Fe-2S]的相关NADH氧化还原酶的鉴定。
Eur J Biochem. 2000 Feb;267(3):666-76. doi: 10.1046/j.1432-1327.2000.01032.x.
6
Biological nitrogen fixation.生物固氮
Annu Rev Nutr. 1993;13:317-35. doi: 10.1146/annurev.nu.13.070193.001533.
7
Spectrophotometric measurement of hydroxylamine and its O-alkyl derivatives.羟胺及其O-烷基衍生物的分光光度测定。
Anal Biochem. 1981 Jan 15;110(2):251-7. doi: 10.1016/0003-2697(81)90143-3.
8
Measurement of protein using bicinchoninic acid.使用二辛可宁酸测定蛋白质。
Anal Biochem. 1985 Oct;150(1):76-85. doi: 10.1016/0003-2697(85)90442-7.
9
Nickel is required for the transfer of electrons from carbon monoxide to the iron-sulfur center(s) of carbon monoxide dehydrogenase from Rhodospirillum rubrum.镍是红螺菌一氧化碳脱氢酶将电子从一氧化碳转移至铁硫中心所必需的。
Biochemistry. 1989 Jun 13;28(12):4968-73. doi: 10.1021/bi00438a010.
10
Characterization of the CO oxidation/H2 evolution system of Rhodospirillum rubrum. Role of a 22-kDa iron-sulfur protein in mediating electron transfer between carbon monoxide dehydrogenase and hydrogenase.深红红螺菌一氧化碳氧化/氢气产生系统的特性。一种22 kDa铁硫蛋白在介导一氧化碳脱氢酶和氢化酶之间电子传递中的作用。
J Biol Chem. 1991 Sep 25;266(27):18395-403.