Liu Yue, Tang Yingping, Ren Shunlin, Chen Lanming
Key Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Shanghai 201306, China.
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
Plants (Basel). 2023 Feb 16;12(4):890. doi: 10.3390/plants12040890.
Infectious diseases caused by pathogenic bacteria severely threaten human health. Traditional Chinese herbs are potential sources of new or alternative medicine. In this study, we analyzed for the first time antibacterial substances in the methanol-phase extract from a traditional Chinese herb- Linn-which showed an inhibition rate of 58.33% against 24 species of common pathogenic bacteria. The extract was further purified using preparative high-performance liquid chromatography (Prep-HPLC), which generated four single fragments (Fragments 1 to 4). The results revealed that Fragment 1 significantly increased bacterial cell surface hydrophobicity and membrane permeability and decreased membrane fluidity, showing disruptive effects on cell integrity of Gram-positive and Gram-negative bacteria, such as , , , and subsp., compared to the control groups ( < 0.05). In sum, 65 compounds with known functions in Fragment 1 were identified using liquid chromatography and mass spectrometry (LC-MS), of which quercetin-3-o-glucuronide was predominant (19.35%). Comparative transcriptomic analysis revealed multiple altered metabolic pathways mediated by Fragment 1, such as inhibited ABC transporters, ribosome, citrate cycle and oxidative phosphorylation, and upregulated nitrogen metabolism and purine metabolism, thereby resulting in the repressed bacterial growth and even death ( < 0.05). Overall, the results of this study demonstrate that Fragment 1 from Linn is a promising candidate against common pathogenic bacteria.
由病原菌引起的传染病严重威胁人类健康。传统中草药是新药或替代药物的潜在来源。在本研究中，我们首次分析了一种传统中草药——林氏草甲醇相提取物中的抗菌物质，该提取物对24种常见病原菌的抑制率为58.33%。使用制备型高效液相色谱（Prep-HPLC）对提取物进一步纯化，得到了四个单一片段（片段1至4）。结果显示，与对照组相比，片段1显著增加了细菌细胞表面疏水性和膜通透性，降低了膜流动性，对革兰氏阳性菌和革兰氏阴性菌（如 、 、 、 亚种）的细胞完整性产生破坏作用（ < 0.05）。总之，利用液相色谱和质谱联用（LC-MS）在片段1中鉴定出65种具有已知功能的化合物，其中槲皮素-3-o-葡萄糖醛酸占主导（19.35%）。比较转录组分析揭示了片段1介导的多种代谢途径改变，如ABC转运蛋白、核糖体、柠檬酸循环和氧化磷酸化受到抑制，氮代谢和嘌呤代谢上调，从而导致细菌生长受到抑制甚至死亡（ < 0.05）。总体而言，本研究结果表明，林氏草的片段1是对抗常见病原菌的一个有前景的候选物。
