Niemi Karoliina, Häggman Hely
Punkaharju Research Station, Finnish Forest Research Institute, 58450 Punkaharju, Finland.
Mycorrhiza. 2002 Oct;12(5):263-7. doi: 10.1007/s00572-002-0181-x. Epub 2002 Jun 20.
The results of the present study show that inoculation with the ectomycorrhizal fungus Pisolithus tinctorius (Pers.) Coker and Couch potentially enhances the germination of Scots pine (Pinus sylvestris L.) somatic embryos in vitro. Stimulation by Pisolithus tinctorius was only observed in the absence of direct contact between the symbionts; mature embryos were not sufficiently robust for balanced interaction with the fungus on half-strength DCR medium. Subsequently, on MMN medium with a reduced sugar concentration, direct contact between somatic embryo-derived plants and the fungus resulted in in vitro formation of mycorrhiza. Ex vitro inoculation also improved adaptation of the somatic embryo-derived plants, even though mycorrhizal structures were not observed. The reactions to Pisolithus tinctorius varied between different Scots pine cell lines both in vitro and ex vitro.
本研究结果表明，接种外生菌根真菌彩色豆马勃（Pisolithus tinctorius (Pers.) Coker and Couch）有可能提高欧洲赤松（Pinus sylvestris L.）体细胞胚的体外萌发率。仅在共生体之间没有直接接触的情况下观察到彩色豆马勃的刺激作用；在半强度DCR培养基上，成熟胚不够健壮，无法与真菌进行平衡的相互作用。随后，在糖浓度降低的MMN培养基上，体细胞胚来源的植株与真菌的直接接触导致了菌根在体外的形成。即使未观察到菌根结构，离体接种也改善了体细胞胚来源植株的适应性。欧洲赤松不同细胞系在体外和离体条件下对彩色豆马勃的反应有所不同。
