Vohník M, Fendrych M, Albrechtová J, Vosátka M
Department of Mycorrhizal Symbioses, Institute of Botany, Academy of Sciences of the Czech Republic, 252 43 Průhonice, Czechia.
Folia Microbiol (Praha). 2007;52(4):407-14. doi: 10.1007/BF02932096.
Four in vitro experiments were set up to verify the colonization potential of ectomycorrhizal (EcM) Cenococcum geophilum FR. (strain CGE-4), saprotrophic Geomyces pannorum (LINK) SIGLER & CARMICHAEL (GPA-1) and a frequent root-associated, potentially ericoid mycorrhiza (ErM)-forming Meliniomyces variabilis Hambleton & Sigler (MVA-1) in roots of Rhododendron and Vaccinium. A typical ErM fungus, Rhizoscyphus ericae (Read) Zhuang & Korf (RER-1), was included for comparison. All fungal strains intracellularly colonized rooted Vaccinium microcuttings: GPA-1 occasionally produced hyphal loops similar to ErM, MVA-1 and RER-1 exhibited a typical ErM colonization pattern. CGE-4 hyphae grew vigorously on and around newly formed roots and rarely penetrated turgescent rhizodermal cells forming intracellular loose loops. Rooting of Rhododendron sp. microcuttings was not promoted by any fungal strain except CGE-4, which also promoted the most vigorous growth of Rhododendron ponticum L. seedlings. The widespread EcM fungus C. geophilum has a potential to colonize non-EcM roots and support their development which may influence overall growth of ericaceous plants. As shown for G. pannorum, structures resembling ErM may be formed by fungi that are to date not regarded as ericoid mycorrhizal.
开展了四项体外实验，以验证外生菌根真菌土生空团菌FR.（菌株CGE - 4）、腐生真菌盘多毛孢菌（LINK）SIGLER & CARMICHAEL（GPA - 1）以及一种常见的与根系相关、可能形成石楠类菌根（ErM）的变孢美柱菌Hambleton & Sigler（MVA - 1）在杜鹃和越橘根系中的定殖潜力。为作比较，纳入了一种典型的石楠类菌根真菌欧石楠类根菌（Read）Zhuang & Korf（RER - 1）。所有真菌菌株均在生根的越橘嫩枝内定殖：GPA - 1偶尔产生类似于石楠类菌根的菌丝环，MVA - 1和RER - 1呈现典型的石楠类菌根定殖模式。CGE - 4的菌丝在新形成的根上及其周围旺盛生长，很少穿透膨胀的根表皮细胞形成细胞内松散环。除CGE - 4外，任何真菌菌株均未促进杜鹃属植物嫩枝生根，而CGE - 4还促进了西洋杜鹃幼苗最旺盛的生长。广泛分布的外生菌根真菌土生空团菌有定殖非外生菌根根系并支持其发育的潜力，这可能会影响石楠科植物的整体生长。如盘多毛孢菌所示，迄今不被视为石楠类菌根的真菌可能会形成类似于石楠类菌根的结构。
