在改良添加剂溶液中储存的红细胞中，2,3-二磷酸甘油酸（2,3 DPG）和三磷酸腺苷（ATP）的维持情况得到改善。

Improved maintenance of 2,3 DPG and ATP in RBCs stored in a modified additive solution.

作者信息

Högman C F, Knutson F, Lööf H, Payrat J M

机构信息

Department of Clinical Immunology and Transfusion Medicine, University Hospital, Uppsala, Sweden.

出版信息

Transfusion. 2002 Jul;42(7):824-9. doi: 10.1046/j.1537-2995.2002.00148.x.

DOI:10.1046/j.1537-2995.2002.00148.x

PMID:12375653

Abstract

BACKGROUND

All currently used systems for the storage of RBCs result in loss of 2,3 DPG and an associated increase in affinity for oxygen. Previously, it was demonstrated that a hypotonic additive solution for RBC storage (Erythro-Sol) resulted in prolonged maintenance of 2,3 DPG when blood was collected in 0.5 CPD (half-strength CPD), but not when full-strength CPD was used. The present study aims at improving the quality of stored RBCs collected in ordinary CPD.

STUDY DESIGN AND METHODS

A new formulation of Erythro-Sol (Erythro-Sol 2) (pH 8.8) in a larger volume (150 mL) was compared with Erythro-Sol (Erythro-Sol 1). In vitro measures during 49 days of storage in the two additives were compared using WBC-depleted RBCs after whole-blood collection in CPD and separation in an automated blood separation instrument (Optipress II, Baxter Healthcare).

RESULTS

The maintenance of RBC ATP and 2,3 DPG was significantly better in Erythro-Sol 2 than in Erythro-Sol 1. The ATP concentration rose to approximately 30 percent above initial level in both systems; however, the maximum occurred on Day 21 in Erythro-Sol 2 as compared with Day 14 in Erythro-Sol 1. In RBCs stored in Erythro-Sol 2, the mean RBC 2,3 DPG concentration increased to 14 percent above initial level on Day 7, then decreased to the initial level on Day 14, whereas in Erythro-Sol 1, the 2,3 DPG had decreased to 85 and 50 percent on Days 7 and 14, respectively. Both intracellular pH and extracellular pH were slightly higher in Erythro-Sol 2 than in Erythro-Sol 1 units but decreased rapidly during the first storage week, which seems to have been the major reason for the limitation in the time of maintenance of 2,3 DPG. Hemolysis was very low in both systems, 0.14 to 0.17 percent on Day 49. The additional amount of inorganic phosphate submitted with Erythro-Sol 2 did not raise concern because the phosphate content in the storage medium, being 1.3 +/- 0.2 mmoL on Day 0, decreased to values below 1 mmoL during most of subsequent storage.

CONCLUSION

Erythro-Sol 2 is an improved additive solution for the storage of RBCs.

摘要

背景

目前所有用于储存红细胞的系统都会导致2,3 - 二磷酸甘油酸（2,3 DPG）的损失以及对氧气亲和力的相应增加。此前已证明，用于红细胞储存的低渗添加剂溶液（Erythro - Sol）在血液采集于0.5 CPD（半强度CPD）时能使2,3 DPG维持更长时间，但使用全强度CPD时则不然。本研究旨在提高普通CPD采集的储存红细胞的质量。

研究设计与方法

将更大体积（150 mL）的新配方Erythro - Sol（Erythro - Sol 2，pH 8.8）与Erythro - Sol（Erythro - Sol 1）进行比较。在CPD中采集全血并通过自动血液分离仪器（Optipress II，百特医疗）分离后，使用去除白细胞的红细胞比较两种添加剂在49天储存期内的体外测量结果。

结果

Erythro - Sol 2中红细胞ATP和2,3 DPG的维持情况明显优于Erythro - Sol 1。在两个系统中，ATP浓度均升至初始水平以上约30%；然而，Erythro - Sol 2在第21天达到最大值，而Erythro - Sol 1在第14天达到最大值。在Erythro - Sol 2中储存的红细胞，平均红细胞2,3 DPG浓度在第7天增加至初始水平以上14%，然后在第14天降至初始水平，而在Erythro - Sol 1中，2,3 DPG在第7天和第14天分别降至85%和50%。Erythro - Sol 2中的细胞内pH和细胞外pH均略高于Erythro - Sol 1，但在储存的第一周迅速下降，这似乎是2,3 DPG维持时间受限的主要原因。两个系统中的溶血率都非常低，在第49天为0.14%至0.17%。随Erythro - Sol 2加入的额外无机磷酸盐量无需担忧，因为储存介质中的磷酸盐含量在第0天为1.3±0.2 mmol，在随后的大部分储存期内降至1 mmol以下。