Huang Hua, Zhou Yu-Feng, Jiang Pei-Zhou, Shen Xin-Ming, Yao Kai-Tai
Institute of Oncology, First Military Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2002 Jul;22(7):602-4.
To screen single nucleotide polymorphisms (SNPs) of 4 human genes at 6p21.3 by way of denaturing high-performance liquid chromatography (DHPLC).
Four exons and 1 intron fragments in the PPP1R11, PPP1R10, FLOT1 and KIAA0170 genes at 6p21.3 isolated from the blood samples from 30 patients with nasopharyngeal carcinoma (NPC) and 28 healthy subjects were amplified by PCR, and the products analyzed by DHPLC. Some fragments of interest were sequenced and compared with the sequences available in National Center for Biotechnology Information (NCBI) database.
In the 5 fragments, 4 new SNPs were identified and 4 known SNP loci and genotypes were confirmed.
DHPLC is an effective, economical, and simple method with reliability for SNPs screening.
通过变性高效液相色谱法（DHPLC）筛选6p21.3区域4个人类基因的单核苷酸多态性（SNP）。
从30例鼻咽癌（NPC）患者和28例健康受试者的血液样本中分离出6p21.3区域PPP1R11、PPP1R10、FLOT1和KIAA0170基因的4个外显子和1个内含子片段，经聚合酶链反应（PCR）扩增，产物用DHPLC分析。对一些感兴趣的片段进行测序，并与美国国立生物技术信息中心（NCBI）数据库中的序列进行比较。
在5个片段中，鉴定出4个新的SNP，确认了4个已知的SNP位点和基因型。
DHPLC是一种有效、经济、简便且可靠的SNP筛选方法。
