高效液相色谱法测定花椒中L-芝麻素和L-细辛脂素的含量
Determination of L-sesamin and L-asarinin in Zanthoxylum(Roxb.) DC. by high performance liquid chromatography.
作者信息
Zhang Shou-Yao, Zhou Ben-Jie, Wang Yan
机构信息
Department of Pharmaceutics, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
出版信息
Di Yi Jun Yi Da Xue Xue Bao. 2002 Jul;22(7):654-5.
OBJECTIVE
To establish a method for determining the content of L-sesamin and L-asarinin indifferent parts of Zanthoxylum(Roxb.)DC.
METHOD
High-performance liquid chromatography (HPLC) was utilized for this purpose with the column of HYPERSIL BDS C18 and the mobile phase containing acetonitrile and water (50:50), the detection wavelength being 287 nm.
RESULTS
The average recovery rates for L-sesamin and L-asarinin were 100.13% and 1.61% and their relative standard deviation (RSD) were 101.24% and 1.46% respectively.
CONCLUSION
The method can be used for the quality control of Zanthoxylum(Roxb.)DC, and the roots of this herb contains more L-sesamin and L-asarinin than its other parts do.
目的
建立一种测定毛叶花椒不同部位中L-芝麻素和L-细辛脂素含量的方法。
方法
采用高效液相色谱法，以HYPERSIL BDS C18柱为色谱柱，以乙腈和水（50:50）为流动相，检测波长为287nm。
结果
L-芝麻素和L-细辛脂素的平均回收率分别为100.13%和1.61%，其相对标准偏差（RSD）分别为101.24%和1.46%。
结论
该方法可用于毛叶花椒的质量控制，该药材根部所含L-芝麻素和L-细辛脂素比其他部位多。
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