[张燕明分散片的制备工艺及葛根素的高效液相色谱法测定]
[Preparation technology of Zhangyanming dispersible tablet and determination of puerarin by HPLC].
作者信息
Zhu Cai-Qing, Li Yan, Di Wu, He Nan-Sheng, Zeng Xian-Yi, Yu Hua
机构信息
Laboratory of Natural Drug, Institute of Materia Medica of Jiangxi Province, Nanchang 330029, China.
出版信息
Zhong Yao Cai. 2009 May;32(5):788-91.
OBJECTIVE
To study the extraction and pharmceutics technology of Zhangyanming dispersible tablet and establish the method for determining the content of puerarin by HPLC.
METHODS
Using total extracted puerarin and disintegrating time as evaluation indexes and the conditions for determination were as follows: Hypersil BDS C18 column (5 microm, 4.6 mm x 200 mm), Methanol-H2O (25:75) as the mobile phase, the detection wavelength at 250 nm, the column temperature at 30 degrees C, and 1.0 mL/min as the mobile phase speed.
RESULTS
The calibration curve was linear at the range of 0.1699 to 1.3594 microg for the puerarin content and the linear equation was y = 3.054 x 10(6)x - 24019 (r = 0.9998). The average recovery ratio was 100.03%, and the relative standard deviation was 1.79%.
CONCLUSIONS
The new preparation technology is reasonable and HPLC can be used to determine the content of puerarin in Zhangyanming dispersible tablet because the operation was convenient, fast, easy to repeat, and the analysis results were accurate and especial.
目的
研究障眼明分散片的提取及制剂工艺，并建立高效液相色谱法测定其中葛根素含量的方法。
方法
以葛根素总提取量和崩解时限为评价指标，测定条件如下：Hypersil BDS C18柱（5μm，4.6mm×200mm），甲醇-水（25:75）为流动相，检测波长250nm，柱温30℃，流动相流速1.0mL/min。
结果
葛根素含量在0.1699～1.3594μg范围内线性关系良好，线性方程为y = 3.054×10(6)x - 24019（r = 0.9998）。平均回收率为100.03%，相对标准偏差为1.79%。
结论
新工艺合理，高效液相色谱法可用于测定障眼明分散片中葛根素的含量，该方法操作简便、快速、重复性好，分析结果准确、特异。
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