高效液相色谱-荧光检测器法测定红三叶草提取物中的苯并芘
[Determination of benzopyrene in red clover extract by high performance liquid chromatography with spectrofluorescence detector].
作者信息
Yan Fu-Hua, Chen Bo, Zeng Jian-Guo, Miao Jian-Rong
机构信息
Key Laboratory of Biology & Traditional Chinese Medicine Research ( Hunan Normal University), Ministry of Education, Hunan Normal University, Changsha 410081, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2007 May;32(10):935-7.
OBJECTIVE
To establish a high performance liquid chromatography method with spectrofluorescence detector for the quantitative analysis of benzopyrene in red clover extract.
METHOD
Analysis was performed on Hypersil C18 column (4. 6 mm x 200 mm, 5 microm) with acetonitrile and water (70: 30) as mobile phase; The excitation wavelength and emission wavelengh were 368 nm and 405 nm, respectively; Flow rate was 1.0 mL x min(-1).
RESULT
The linear range was 0.93-37.2 ng x mL(-1), and r = 0.9992 (n = 5). The average recovery of benzopyrene was 85.2% (n = 9).
CONCLUSION
The method was convenient and accurate with good recovery.
目的
建立一种采用荧光检测器的高效液相色谱法，用于定量分析红三叶草提取物中的苯并芘。
方法
采用Hypersil C18柱（4.6 mm×200 mm，5μm）进行分析，以乙腈和水（70:30）为流动相；激发波长和发射波长分别为368 nm和405 nm；流速为1.0 mL·min⁻¹。
结果
线性范围为0.93 - 37.2 ng·mL⁻¹，r = 0.9992（n = 5）。苯并芘的平均回收率为85.2%（n = 9）。
结论
该方法简便、准确，回收率良好。
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