Berezikov Eugene, Plasterk Ronald H A, Cuppen Edwin
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.
Bioinformatics. 2002 Oct;18(10):1396-7. doi: 10.1093/bioinformatics/18.10.1396.
GENOTRACE identifies the genomic organization for a cDNA using raw data from genome sequencing projects in progress (trace archives). Local genomic contigs are generated, allowing for example the design of PCR primers in intronic sequences to amplify coding regions of a gene, needed for example for mutation or SNP detection.
The package and examples of output files can be downloaded from http://rat.niob.knaw.nl/GENOTRACE
GENOTRACE使用来自正在进行的基因组测序项目（序列档案）的原始数据来识别cDNA的基因组组织。生成局部基因组重叠群，例如允许设计位于内含子序列中的PCR引物来扩增基因的编码区，这在例如突变或单核苷酸多态性检测中是必需的。
该软件包和输出文件示例可从http://rat.niob.knaw.nl/GENOTRACE下载。
