Brodie Ryan, Roper Rachel L, Upton Chris
Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC V8W 3P6, Canada.
Bioinformatics. 2004 Jan 22;20(2):279-81. doi: 10.1093/bioinformatics/btg406.
Java-Dotter (JDotter) is a platform-independent Java interactive interface for the Linux version of Dotter, a widely used program for generating dotplots of large DNA or protein sequences. JDotter runs as a client-server application and can send new sequences to the Dotter program for alignment as well as rapidly access a repository of preprocessed dotplots. JDotter also interfaces with a sequence database or file system to display supplementary feature data. Thus, JDotter greatly simplifies access to dotplot data in laboratories that deal with large numbers of genomes and have a multi-platform organization.
Currently, JDotter is used via Java Web Start by the Poxvirus Bioinformatics Resource for examining dotplots of complete poxvirus genomes; http://athena.bioc.uvic.ca/pbr/jdotter/. The software is available for download from the same location.
Installation instructions, the User's Manual, screenshots and examples are available at the JDotter home page http://athena.bioc.uvic.ca/pbr/jdotter/. The software and source code is free for non-commercial applications.
Java-Dotter(JDotter)是一个独立于平台的Java交互式界面,用于Linux版的Dotter,Dotter是一个广泛用于生成大型DNA或蛋白质序列点阵图的程序。JDotter作为客户端-服务器应用程序运行,可将新序列发送到Dotter程序进行比对,并能快速访问预处理点阵图的存储库。JDotter还与序列数据库或文件系统接口以显示补充特征数据。因此,JDotter极大地简化了在处理大量基因组且具有多平台架构的实验室中访问点阵图数据的过程。
目前,痘病毒生物信息学资源通过Java Web Start使用JDotter来检查完整痘病毒基因组的点阵图;网址为http://athena.bioc.uvic.ca/pbr/jdotter/。该软件可从同一位置下载。
安装说明、用户手册、屏幕截图和示例可在JDotter主页http://athena.bioc.uvic.ca/pbr/jdotter/获取。该软件和源代码对于非商业应用是免费的。