Johne R, Konrath A, Krautwald-Junghanns M-E, Kaleta E F, Gerlach H, Müller H
Institute for Virology, Faculty of Veterinary Medicine, University of Leipzig, Leipzig, Germany.
Arch Virol. 2002 Oct;147(10):1869-80. doi: 10.1007/s00705-002-0858-7.
Internal papillomatosis of parrots (IPP) is a tumour disease with unknown etiology, characterised by progressive development of papillomas in the oral and cloacal mucosa. Based on epidemiologic data, infectious agents, particularly DNA tumour viruses, are considered to be involved. In this study, cloacal papillomas were investigated by PCR for the presence of herpesvirus, papillomavirus and avian polyomavirus genomes, respectively. Using consensus and specific primers, 5 out of 12 papillomas were tested positive for herpesvirus; all papillomas were tested negative for papillomavirus and avian polyomavirus. The DNA sequence of one of the PCR products showed 86.5% homology to the corresponding region of the psittacine herpesvirus 1 DNA polymerase gene. Using a PCR with primers based on this sequence, additional 4 papillomas were tested positive. By in situ hybridisation, herpesviral sequences were detected in epithelial cells of the papilloma, but not in surrounding tissues. As 75% of the tumours proved to be positive, these data suggest an involvement of a herpesvirus in the etiology of IPP; the distinct role, however, needs to be investigated.
鹦鹉内乳头状瘤病（IPP）是一种病因不明的肿瘤疾病，其特征是口腔和泄殖腔黏膜中乳头状瘤逐渐发展。根据流行病学数据，感染因子，特别是DNA肿瘤病毒，被认为与之有关。在本研究中，分别通过PCR检测泄殖腔乳头状瘤中是否存在疱疹病毒、乳头瘤病毒和禽多瘤病毒基因组。使用通用引物和特异性引物，12个乳头状瘤中有5个检测出疱疹病毒呈阳性；所有乳头状瘤检测乳头瘤病毒和禽多瘤病毒均为阴性。其中一个PCR产物的DNA序列与鹦鹉疱疹病毒1 DNA聚合酶基因的相应区域显示出86.5%的同源性。使用基于该序列的引物进行PCR，另外4个乳头状瘤检测呈阳性。通过原位杂交，在乳头状瘤的上皮细胞中检测到疱疹病毒序列，但在周围组织中未检测到。由于75%的肿瘤被证明为阳性，这些数据表明疱疹病毒与IPP的病因有关；然而，其具体作用仍需进一步研究。
