针对传染性法氏囊病病毒中和单克隆抗体的抗独特型抗体不干扰病毒感染。

Anti-idiotypic antibody specific for an infectious bursal disease virus-neutralizing monoclonal antibody does not interfere with the virus infection.

作者信息

Yamaguchi T, Igusa A, Setiyono A, Fukushi H, Hirai K

机构信息

Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, Japan.

出版信息

Arch Virol. 2002 Oct;147(10):2017-23. doi: 10.1007/s00705-002-0864-9.

DOI:10.1007/s00705-002-0864-9

PMID:12376762

Abstract

Infectious bursal disease virus (IBDV) capsid protein, VP2, contains a hypervariable domain that is recognized by virus-neutralizing antibodies. The virus-neutralizing epitope is highly conformation-dependent and the domain is speculated to be involved in the virus-target cell interaction. In this study, a polyclonal anti-idiotypic antibody (anti-id) was generated by the sequential immunization of a rabbit with a virus-neutralizing monoclonal antibody GI-11 which recognizes the VP2 hypervariable domain. Although the anti-id, which mimics the conformational epitope in the VP2 hypervariable domain, was expected to inhibit the virus infection, the anti-id did not interfere with either the virus binding or the infection to the target cell.

摘要

传染性法氏囊病病毒（IBDV）的衣壳蛋白VP2包含一个高变区，该区域可被病毒中和抗体识别。病毒中和表位高度依赖构象，推测该区域参与病毒与靶细胞的相互作用。在本研究中，通过用识别VP2高变区的病毒中和单克隆抗体GI-11对兔子进行连续免疫，产生了一种多克隆抗独特型抗体（抗Id）。尽管预期模拟VP2高变区构象表位的抗Id会抑制病毒感染，但该抗Id并未干扰病毒与靶细胞的结合或感染。