Melrose James, Smith Susan, Knox Sarah, Whitelock John
The Institute of Bone and Joint Research, The University of Sydney, Department of Surgery, The University Clinic, Royal North Shore Hospital of Sydney, St. Leonards, NSW, Australia.
Histochem Cell Biol. 2002 Oct;118(4):269-80. doi: 10.1007/s00418-002-0449-4. Epub 2002 Sep 12.
The aim of this study was to immunolocalise perlecan in ovine vertebral growth plate (VGP) and cartilaginous endplate (CEP) cartilages using a monoclonal antibody (MAb A76) directed to a core protein epitope in perlecan domain-I, and to compare and contrast its localisation patterns with known cartilage matrix components. Perlecan was a prominent pericellular component of mature hypertrophic chondrocytes in the VGP and CEP in newborn 2- to 5-day-old sheep. Type I, II, VI and X collagen, chondroitin-4 and 6-sulphate, 7-D-4 chondroitin sulphate isomer proteoglycan epitope, keratan sulphate, aggrecan core protein, hyaluronan (HA) and hyaluronan binding proteins (HABPs) each had distinct localisation patterns in the VGP and CEP. Type X collagen was a prominent component of the VGP but was undetectable in the CEP. Aggrecan was strongly localised extracellularly throughout the VGP and CEP but increased cell-associated staining was also evident. In contrast to the aforementioned matrix components, HA, HABPs and perlecan were localised strongly to the pericellular matrices of the hypertrophic VGP and CEP chondrocytes apparently indicating an important role for these components in terminal chondrocyte differentiation.
本研究的目的是使用针对核心蛋白聚糖结构域I中一个核心蛋白表位的单克隆抗体（单克隆抗体A76），对绵羊椎体生长板（VGP）和软骨终板（CEP）软骨中的核心蛋白聚糖进行免疫定位，并将其定位模式与已知的软骨基质成分进行比较和对比。在出生2至5天的新生绵羊的VGP和CEP中，核心蛋白聚糖是成熟肥大软骨细胞的一种显著的细胞周成分。I型、II型、VI型和X型胶原蛋白、硫酸软骨素-4和-6、7-D-4硫酸软骨素异构体蛋白聚糖表位、硫酸角质素、聚集蛋白聚糖核心蛋白、透明质酸（HA）和透明质酸结合蛋白（HABP）在VGP和CEP中各自具有不同的定位模式。X型胶原蛋白是VGP的一种主要成分，但在CEP中未检测到。聚集蛋白聚糖在整个VGP和CEP的细胞外强烈定位，但细胞相关染色增加也很明显。与上述基质成分相反，HA、HABP和核心蛋白聚糖强烈定位于肥大的VGP和CEP软骨细胞的细胞周基质，这显然表明这些成分在终末软骨细胞分化中起重要作用。
