Meng Hui, Gao Ling, Shen Hong, Chen Gang, Cai Rui-Jun, Mu Feng, Zhao Fei
Department of Cardiothoracic Surgery, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2002 Oct;22(10):898-901.
To investigate the structural changes in canine lungs perfused with modified Euro-Collins solution (mECS), thus providing insight into the preservation of the donor lung tissue for transplantation.
Six dogs were anesthetized and pulmonary perfusion with mECS (4 degrees Celsius) was performed before the lungs were isolated thereafter and stored at 4 to 8 degrees Celsius for 30, 60, 120, 180, 240 min respectively, after which tissue samples of the donor lung were taken for morphological observation with both light and transmission electron microscope.
Immediately after reperfusion, the pulmonary tissues exhibited clear and intact structures,showing that mitochondrion swelled slightly in the alveolar epithelial cells (AECs). Mild edema occurred in the alveolar wall and the tissues around the veins at 30 min after preservation, which exacerbated to dilapidation of the alveolar wall and obvious tumefaction of the mitochondrion in the AECs at 60 min. At 180 min, rupture of alveolar wall and emergence of the alveolus was observed at 120 min, and a few pulmonary bullae were formed, the AECs (I) of which presented vacuolar changes in the cytoplasma and mitochondrion, with disappearance of the tiny villus of the AECs (II) at 240 min.
Within 4 h of preservation in mECS, the pulmonary tissues do not undergo obvious changes as signs of injury, which, however, may not be the case after longer preservation.
研究用改良欧洲柯林斯溶液（mECS）灌注的犬肺的结构变化，从而深入了解供体肺组织移植保存情况。
对6只犬进行麻醉，在分离肺脏前用mECS（4摄氏度）进行肺灌注，之后分别在4至8摄氏度下保存30、60、120、180、240分钟，然后取供体肺组织样本，用光学显微镜和透射电子显微镜进行形态学观察。
再灌注后即刻，肺组织结构清晰完整，显示肺泡上皮细胞（AECs）中的线粒体轻度肿胀。保存30分钟后，肺泡壁和静脉周围组织出现轻度水肿，60分钟时水肿加剧，肺泡壁破损，AECs中的线粒体明显肿胀。120分钟时观察到肺泡壁破裂和肺泡出现，180分钟时形成一些肺大疱，240分钟时肺大疱的AECs（I）细胞质和线粒体出现空泡样改变，AECs（II）的微绒毛消失。
在mECS中保存4小时内，肺组织未出现明显的损伤迹象，但保存时间更长时可能并非如此。
