Quan Song, Zhou Hai-Kuan, Shuji Yamano, Hisayo Nakasaka, Toshihiro Aono
Department of Obstetrics and Gynecology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2002 Oct;22(10):928-30.
To assess the fertilizing capacity of human sperm preserved in electrolyte-free (EF) solution at 4 degrees Celsius.
The motility, acrosome status and fertility index of human sperm were respectively assessed before and after preservation in cold EF solution.
The motility of human sperm so preserved for one week was significantly higher than that of the sperm preserved in modified human tubal fluid (43.4+/-7.9% vs 9.5+/-2.5%, P<0.01). Although acrosome status of human sperm in EF solution before reinitiation differed little from that of fresh sperm (capacitated sperm: 7.6+/-1.8% vs 6.4+/-1.8%; acrosome-reacted sperm: 3.0+/-1.7% vs 2.4+/-1.1%, P>0.05), the percentage of capacitated and acrosome-reacted sperm in the EF solution significantly increased after reinitiation (16.0+/-2.3% vs 7.6+/-1.8% and 9.4+/-2.1% vs 3.0+/-1.7% respectively, P<0.01). The penetration rate and fertility index of the sperm in EF solution were comparable with those of fresh sperm (48.1% vs 50.9%; 1.38+/-0.16 vs 1.29+/-0.13, respectively).
Preservation in cold EF solution does not induce human sperm to undergo capacitation and acrosome reaction, and the sperm so preserved for one week possesses comparable fertilizing capacity as fresh sperm does.
评估人类精子在无电解质（EF）溶液中于4摄氏度保存后的受精能力。
分别评估人类精子在冷EF溶液中保存前后的活力、顶体状态和受精指数。
如此保存一周的人类精子活力显著高于保存在改良人类输卵管液中的精子（43.4±7.9%对9.5±2.5%，P<0.01）。尽管重新开始实验前EF溶液中人类精子的顶体状态与新鲜精子的顶体状态差异不大（获能精子：7.6±1.8%对6.4±1.8%；顶体反应精子：3.0±1.7%对2.4±1.1%，P>0.05），但重新开始实验后EF溶液中获能和顶体反应精子的百分比显著增加（分别为16.0±2.3%对7.6±1.8%和9.4±2.1%对3.0±1.7%，P<0.01）。EF溶液中精子的穿透率和受精指数与新鲜精子相当（分别为48.1%对50.9%；1.38±0.16对1.29±0.13）。
在冷EF溶液中保存不会诱导人类精子发生获能和顶体反应，如此保存一周的精子具有与新鲜精子相当的受精能力。
